Abstract

We plan to study the genetics of antigen recognition in T lymphocytes using T cell hybridomas, anti-receptor antibodies and DNA probes for receptor genes. Our general approach is to examine the V genes in hybridomas of known specificity and those of related specificity in order to correlate receptor structure with Ag/MHC recognition. Specific projects include mapping the beta locus on chromosome 6, examination of V genes in hybridomas which recognize a conventional antigen (ovalbumin) or an alloantigen (I-Ab), or a self-antigen (Dd). We will plan to sequence an alpha chain polypeptide in order to clone genes in this locus. Finally, we propose to examine receptor expression in the developing thymus. Ultimately, we hope to better understand how Ag/MHC recognition is encoded in the genome.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI022259-03
Application #
3133165
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1985-09-01
Project End
1988-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
National Jewish Health
Department
Type
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80206

  Publications

Ignatowicz, L; Kappler, J W; Marrack, P et al. (1994) Identification of two V beta 7-specific viral superantigens. J Immunol 152:65-71
Marrack, P; Hugo, P; McCormack, J et al. (1993) Death and T cells. Immunol Rev 133:119-29
Marrack, P; Winslow, G M; Choi, Y et al. (1993) The bacterial and mouse mammary tumor virus superantigens;two different families of proteins with the same functions. Immunol Rev 131:79-92
Hugo, P; Kappler, J W; Marrack, P C (1993) Positive selection of TcR alpha beta thymocytes: is cortical thymic epithelium an obligatory participant in the presentation of major histocompatibility complex protein? Immunol Rev 135:133-55
Pullen, A M; Choi, Y; Kushnir, E et al. (1992) The open reading frames in the 3' long terminal repeats of several mouse mammary tumor virus integrants encode V beta 3-specific superantigens. J Exp Med 175:41-7
Woodland, D L; Lund, F E; Happ, M P et al. (1991) Endogenous superantigen expression is controlled by mouse mammary tumor proviral loci. J Exp Med 174:1255-8
Pullen, A M; Bill, J; Kubo, R T et al. (1991) Analysis of the interaction site for the self superantigen Mls-1a on T cell receptor V beta. J Exp Med 173:1183-92
Herman, A; Croteau, G; Sekaly, R P et al. (1990) HLA-DR alleles differ in their ability to present staphylococcal enterotoxins to T cells. J Exp Med 172:709-17
Pullen, A M; Wade, T; Marrack, P et al. (1990) Identification of the region of T cell receptor beta chain that interacts with the self-superantigen MIs-1a. Cell 61:1365-74
Marrack, P; Blackman, M; Kushnir, E et al. (1990) The toxicity of staphylococcal enterotoxin B in mice is mediated by T cells. J Exp Med 171:455-64

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