The available chlamydial genome sequences have made it possible to study chlamydia at the whole genome scale. For example, comparison of genome sequences have revealed functions of chlamydial genes with homologies to their counterparts in other species and provided information for chlamydial evolutionary history. Genome wide analyses of gene transcriptional profiles have correlated gene expression patterns with biological responses. However, these nucleic acid sequence-based analyses have limitations in fully understanding the functions of chlamydial proteins. Due to a lack of genetic manipulation for chlamydia, researchers are forced to develop function-driven genome wide screening assays in heterologous systems. While some of these assays have yielded useful information, many have met little success. To complement the above approaches for identifying functions of chlamydial proteins, we are proposing a functional proteomics approach, in which whole genome protein array assays will be developed for identifying protective and pathogenic determinants during human chlamydial infection and determining protein-protein interaction pairs. Furthermore, antibodies will be produced for characterizing endogenous chlamydial proteins for expression levels/patterns, half-life, post-translational modifications, interaction partners and intracellular localization. These proposed studies will provide essential information for understanding chlamydial pathogenic mechanisms and developing intervention and prevention strategies for controlling chlamydial infection-induced diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI064537-02
Application #
7037624
Study Section
Host Interactions with Bacterial Pathogens Study Section (HIBP)
Program Officer
Hiltke, Thomas J
Project Start
2005-04-01
Project End
2009-12-31
Budget Start
2006-01-01
Budget End
2006-12-31
Support Year
2
Fiscal Year
2006
Total Cost
$356,423
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Microbiology/Immun/Virology
Type
Other Domestic Higher Education
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
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Flores, Rhonda; Zhong, Guangming (2015) The Chlamydia pneumoniae Inclusion Membrane Protein Cpn1027 Interacts with Host Cell Wnt Signaling Pathway Regulator Cytoplasmic Activation/Proliferation-Associated Protein 2 (Caprin2). PLoS One 10:e0127909
Yang, Zhangsheng; Tang, Lingli; Sun, Xin et al. (2015) Characterization of CPAF critical residues and secretion during Chlamydia trachomatis infection. Infect Immun 83:2234-41
Chen, Jianlin; Yang, Zhangsheng; Sun, Xin et al. (2015) Intrauterine infection with plasmid-free Chlamydia muridarum reveals a critical role of the plasmid in chlamydial ascension and establishes a model for evaluating plasmid-independent pathogenicity. Infect Immun 83:2583-92

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