Abstract

The use of animal viruses as model systems for probing the complexities of molecular control mechanisms has been particularly fruitful. It is generally throught that the understanding of genetic regulation in viruses will provide an insight into similar processes in eucaryotic cells. The overall objective of our research is to systematically develop our understanding of gene regulation in virus infected cells. This knowledge will be used for studying the regulation of normal cellular genes and oncogenes and for developing in vitro systems from which activities can be purified and characterized. In the present revised renewal application we especially intend to accomplish the following: 1. To characterize the structure of actively transcribed SV40 minichromosomes. 2. To study the mechanism of transcription termination at the attenuation site. 3. To study attenuation as a mechanism regulating viral and cellular gene expression (globin and c-myc). 4. To purify and characterize cellular termination and readthrough factors. 5. To study the significance of RNA secondary structure in the regulation of eucaryotic gene expression. 6. To study the role of agnoprotein in regulating SV40 gene expression and in virus assembly. The acquisition of knowledge by the present research will ultimately contribute to a better understanding of the more complex phenomena in mammalian cells, such as the molecular processes of differentiation, development and malignant transformation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA014995-13A1
Application #
3164056
Study Section
Virology Study Section (VR)
Project Start
1977-03-01
Project End
1990-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
13
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Weizmann Institute of Science
Department
Type
DUNS #
City
Rehovot
State
Country
Israel
Zip Code
76100

  Publications

Shor, J; Ben-Asher, E; Aloni, Y (1995) Transcription elongation of the murine ornithine decarboxylase (ODC) gene is regulated in vitro at two downstream elements by different attenuation mechanisms. Oncogene 10:1587-96
Ori, A; Shaul, Y (1995) Hepatitis B virus enhancer binds and is activated by the Hepatocyte nuclear factor 3. Virology 207:98-106
Krauskopf, A; Aloni, Y (1994) A cellular repressor regulates transcription initiation from the minute virus of mice P38 promoter. Nucleic Acids Res 22:828-34
Krauskopf, A; Ben-Asher, E; Aloni, Y (1994) Minute virus of mice infection modifies cellular transcription elongation. J Virol 68:2741-5
Levine, A; Yeivin, A; Ben-Asher, E et al. (1993) Histone H1-mediated inhibition of transcription initiation of methylated templates in vitro. J Biol Chem 268:21754-9
Goldring, N B; Kessler, M; Aloni, Y (1992) Parameters affecting the elongation block by RNA polymerase II at the SV40 attenuator 1 in vitro. Biochemistry 31:8369-76
Usheva, A; Maldonado, E; Goldring, A et al. (1992) Specific interaction between the nonphosphorylated form of RNA polymerase II and the TATA-binding protein. Cell 69:871-81
Resnekov, O; Pruzan, R; Aloni, Y (1991) Elements involved in an in vitro block to transcription elongation at the end of the L1 mRNA family of adenovirus 2. Nucleic Acids Res 19:1783-90
Bengal, E; Flores, O; Krauskopf, A et al. (1991) Role of the mammalian transcription factors IIF, IIS, and IIX during elongation by RNA polymerase II. Mol Cell Biol 11:1195-206
Krauskopf, A; Bengal, E; Aloni, Y (1991) The block to transcription elongation at the minute virus of mice attenuator is regulated by cellular elongation factors. Mol Cell Biol 11:3515-21

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