Structure and Function of SV40 Non-Virion Proteins

Livingston, David Morse

Abstract

We are engaged in a coordinated study of the structure and function of the SV40 large T antigen. In progress are experiments designed to assess 1) the role of the two enzymatic activities associated with SV40 T antigen - ATP'ase and protein kinase; 2) the nature of non-origin region SV40 DNA binding sites; 3) the role of large T in the induction of the 55K cellular Tau protein; 4) the structure of the carboxy terminus of this protein isolated from both transformed and lytically infected cells and 5) the sites of T antigen binding in cellular DNA.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project (R01)
• Project #: 5R01CA015751-12
• Application #: 3164265
• Study Section: Experimental Virology Study Section (EVR)
• Project Start: 1981-09-01
• Project End: 1986-08-31
• Budget Start: 1985-09-01
• Budget End: 1986-08-31
• Support Year: 12
• Fiscal Year: 1985

Institution

• Name: Dana-Farber Cancer Institute
• DUNS #: 149617367
• City: Boston
• State: MA
• Country: United States

Publications

Pretto, D; Barco, R; Rivera, J et al. (2006) The synovial sarcoma translocation protein SYT-SSX2 recruits beta-catenin to the nucleus and associates with it in an active complex. Oncogene 25:3661-9

Grossman, Steven R; Deato, Maria E; Brignone, Chrystelle et al. (2003) Polyubiquitination of p53 by a ubiquitin ligase activity of p300. Science 300:342-4

Kaelin Jr, W G; Ewen, M E; Livingston, D M (1990) Definition of the minimal simian virus 40 large T antigen- and adenovirus E1A-binding domain in the retinoblastoma gene product. Mol Cell Biol 10:3761-9

Murphy, C I; Weiner, B; Bikel, I et al. (1988) Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells. J Virol 62:2951-9

Brown, M; McCormack, M; Zinn, K G et al. (1986) A recombinant murine retrovirus for simian virus 40 large T cDNA transforms mouse fibroblasts to anchorage-independent growth. J Virol 60:290-3

Bikel, I; Mamon, H; Brown, E L et al. (1986) The t-unique coding domain is important to the transformation maintenance function of the simian virus 40 small t antigen. Mol Cell Biol 6:1172-8

Tenen, D G; Haines, L L; Hansen, U M et al. (1985) Formation of a cruciform structure at the simian virus 40 replication origin abolishes T-antigen binding to the origin in vitro. J Virol 56:293-7