We propose to continue our ongoing work on the functional properties of the SV40 large T antigen (T), one of the two transforming proteins of the virus. Attention will be focused on the biological significance and the mechanism underlying the large T adenylation phenomenon. Moreover, we will attempt to develop tractable assays for the neoplastic transforming activity of purified T and, then, to use these to assay for this function in cells injected with various mutant and chemically modified wt T species. The major goal here is to understand the structure-function relationships underlying the large T transforming function. Finally, a parallel effort will be made to elucidate those key structure-function relationships which govern the in vitro biochemical activities of the protein. One of the purposes of this particular work is to begin to understand their physiologic significance and how they contribute to the in vivo behavior of this protein in both the lytic cycle and in SV40 transformed cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA015751-15
Application #
3164267
Study Section
Experimental Virology Study Section (EVR)
Project Start
1981-09-01
Project End
1991-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
15
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02115