The interaction of parvovirus with its host cell is being used as a probe of eukaryotic cell differentiation, DNA replication, and aging. We will explore the properties of parvovirus early and late promoters and the interaction between them. The early promoter produces a gene product that positively regulates the late promoter. The mechanisms and sequences involved in this trans-activation will be studied. The parvovirus early promoter will be tested for dependence on a host cell regulatory protein and cell lines suitable for cloning the gene for such a protein isolated. In the terminal stages of fibroblast differentiation, the senescing cells become resistant to parvovirus infection and these cells will be tested for restriction of virus early promoter activity.
| Rhode 3rd, S L; Paradiso, P R (1989) Parvovirus replication in normal and transformed human cells correlates with the nuclear translocation of the early protein NS1. J Virol 63:349-55 |
| Rhode 3rd, S L; Richard, S M (1987) Characterization of the trans-activation-responsive element of the parvovirus H-1 P38 promoter. J Virol 61:2807-15 |
