Chronic kidney disease (CKD) has reached epidemic proportions globally. The single most important killer of CKD patients is cardiovascular disease (CVD) consisting of cardiac hypertrophy and fibrosis, and vascular calcification. More CKD patients die from CVD than reaching the need for dialysis. The treatment of traditional cardiovascular risk factors have met with limited success and we are in dire need to treat CKD-related CVD. Uremic cardiomyopathy is a complex metabolic disease with a panoply of underlying pathophysiologic factors. Our laboratory has focused on contributing mineral factors common to CVD and CKD-Mineral Bone Disorders (MBD) - which individually contributes to uremic cardiomyopathy, but each one also exacerbates the others creating a self-amplifying unrelenting vortex. We focus on the model that Klotho deficiency and phosphotoxicity exacerbate each other and both contribute to CVD. If we disrupt these disturbances simultaneously, it will be much more effective than manipulating them alone thus furnishing a novel and efficacious regimen to prevent and treat uremic cardiomyopathy.
In Aim 1, we will use a well-established rodent model of CKD and uremic cardiomyopathy to test this therapeutic approach. These are empiric but important proof-of-concept studies to secure the utility of this approach.
In Aim 2, we will examine the long sort after but yet unresolved question of the underlying mechanism of Klotho deficiency in CKD. We know that uremia, Klotho deficiency exacerbates phosphotoxicity by reducing phosphaturia but how Klotho is suppressed in CKD is not known. We will test the model that phosphate loading causes systemic Klotho deficiency by at least two mechanisms: direct inhibition of Klotho transcription via methylation of its promoter; and reduced Klotho shedding from the kidney using combined in vivo and in vitro approaches. Although Klotho deficiency per se have been shown to pathologic cardiac remodeling, the molecular mechanism(s) that mediate(s) its action is still an enigma. We constructed a model and propose that one principal mechanism is the balance between autophagy and apoptosis and Klotho holds the ?toggle switch? by modifying the formation of the important autophagy complex Beclin 1/Bcl-2.
In Aim 3, we will use both in vivo and in vitro models to test the paradigm. Using genetically modified animals, we will manipulate autophagy levels and test whether the protective action of Klotho is mediated by enhanced autophagy. We will use in vitro models to further test the model of phosphate loading and Klotho flipping the toggle switch in opposite directions. The proposed studies will uncover some fundamental biologic mechanisms of how Klotho is suppressed in CKD and phosphate loading, elucidate how Klotho protects the cell via autophagy flux, and finally provide the critical pre-clinical data to form the foundation for translation to therapy in human CKD and uremic cardiomyopathy.

Public Health Relevance

Chronic kidney disease (CKD) has reached epidemic proportions globally with the single most important killer of CKD patient being cardiovascular disease, and we have identified that excess phosphate, and deficient Klotho (a circulating protein that protects cells), are major contributors to heart disease and death in CKD in rodent studies. Since these factors individually contribute to heart disease and they also amplify each other, we will treat all both of them simultaneously (double therapy) and see if that slows down CKD progression and ameliorate heart disease and death. We will also undercover some of the molecular mechanisms of how phosphate and klotho therapy works and if these studies are positive, they will pave the way to translate this regimen to treat humans with CKD.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK091392-07
Application #
9751266
Study Section
Pathobiology of Kidney Disease Study Section (PBKD)
Program Officer
Chan, Kevin E
Project Start
2011-04-10
Project End
2022-05-31
Budget Start
2019-06-01
Budget End
2020-05-31
Support Year
7
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
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Chen, Gaozhi; Liu, Yang; Goetz, Regina et al. (2018) ?-Klotho is a non-enzymatic molecular scaffold for FGF23 hormone signalling. Nature 553:461-466
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Lu, Xiang; Hu, Ming Chang (2017) Klotho/FGF23 Axis in Chronic Kidney Disease and Cardiovascular Disease. Kidney Dis (Basel) 3:15-23

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