This research program will investigate the molecular mechanisms which carry out the effects of cyclic AMP and cyclic CMP on the cornea. This includes the localization of beta-adrenergic and muscarinic cholinergic receptors within the corneal epithelium using radioligand binding to isolated subpopulation of basal, winged and superficial eptithelial cells and photoaffinity labeling techniques in situ. The observed cyclic AMP inhibition of collagen and glycosaminoglycan production by bovine keratocytes will be characterized and the mechanism identified. These studies will be extended to other corneal cells (bovine and human) in culture. A comparative biochemical examination of cyclic nucleotide-related enzymes will be made on corneal endothelia of species which have """"""""regenerative"""""""" (rabbit and bovine) vs. """"""""nonregenerative"""""""" (cat and human) endothelial cells. This knowledge will be used to develop drug regimens to pharmacologically stimulate endothelial proliferation in wounded cat and human corneas in organ culture. The prostaglandin synthetic pathways of corneal cells will be examined using a new, simple technique for separation and identification of prostaglandin-like substances.
| Walkenbach, R J; Ye, G S; Korenfeld, M S et al. (1993) Atrial natriuretic peptide receptors on the corneal endothelium. Invest Ophthalmol Vis Sci 34:2538-43 |
| Walkenbach, R J; Ye, G S; Boney, F et al. (1993) Muscarinic cholinoceptors in native and cultured human corneal endothelium. Curr Eye Res 12:155-62 |
| Walkenbach, R J; Ye, G S; Reinach, P S et al. (1992) Alpha 1-adrenoceptors in the corneal endothelium. Exp Eye Res 55:443-50 |
| Walkenbach, R J; Boney, F; Ye, G S (1992) Corneal function after storage in dexsol or optisol. Invest Ophthalmol Vis Sci 33:2454-8 |
| Walkenbach, R J; Ye, G S; Reinach, P S et al. (1991) Alpha 1-adrenoceptors in human corneal epithelium. Invest Ophthalmol Vis Sci 32:3067-72 |
| Walkenbach, R J; Boney, F; Ye, G S (1991) The effects of UW solution and its components on corneal thickness during and after storage. Curr Eye Res 10:1129-36 |
| Walkenbach, R J; Corwin, J G; Ye, G S (1991) Corneal function after storage in commercial eye bank media. Invest Ophthalmol Vis Sci 32:1551-7 |
| Walkenbach, R J; Ye, G S (1991) Muscarinic cholinoceptor regulation of cyclic guanosine monophosphate in human corneal epithelium. Invest Ophthalmol Vis Sci 32:610-5 |
| Walkenbach, R J; Ye, G S (1990) Muscarinic receptors and their regulation of cyclic GMP in corneal endothelial cells. Invest Ophthalmol Vis Sci 31:702-7 |
| Walkenbach, R J; Corwin, J G (1987) Corneal deturgescence during organ culture. Curr Eye Res 6:381-9 |
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