This proposal describes experiments to systematically study the biology of the cornea in vitro. Special emphasis is placed on pharmacological control of corneal deturgescence and a simpler techique to study corneal deturgescence will be employed. The techniques used in the basic research studies of rabbit corneal deturgescence are designed to be equally applicable to use in eye banks to improve both storage and evaluative techniques for human donor eyes. Initial studies seek to improve the present eye bank protocols of corneal storage at 4 degrees. Other studies seek to develop a better corneal organ culture method; one which sustains both rabbit and human corneal function (deturgescence) throughout the culture period. The organ culture technique would be used in studies of the characteristics of collagen and glycoconjugate synthesis by intact rabbit and human corneas in vitro. Studies on corneal endothelial wound healing and its pharmacological regulation will also be performed in the organ culture system.
| Walkenbach, R J; Ye, G S; Boney, F et al. (1993) Muscarinic cholinoceptors in native and cultured human corneal endothelium. Curr Eye Res 12:155-62 |
| Walkenbach, R J; Ye, G S; Korenfeld, M S et al. (1993) Atrial natriuretic peptide receptors on the corneal endothelium. Invest Ophthalmol Vis Sci 34:2538-43 |
| Walkenbach, R J; Ye, G S; Reinach, P S et al. (1992) Alpha 1-adrenoceptors in the corneal endothelium. Exp Eye Res 55:443-50 |
| Walkenbach, R J; Boney, F; Ye, G S (1992) Corneal function after storage in dexsol or optisol. Invest Ophthalmol Vis Sci 33:2454-8 |
| Walkenbach, R J; Ye, G S (1991) Muscarinic cholinoceptor regulation of cyclic guanosine monophosphate in human corneal epithelium. Invest Ophthalmol Vis Sci 32:610-5 |
| Walkenbach, R J; Ye, G S; Reinach, P S et al. (1991) Alpha 1-adrenoceptors in human corneal epithelium. Invest Ophthalmol Vis Sci 32:3067-72 |
| Walkenbach, R J; Boney, F; Ye, G S (1991) The effects of UW solution and its components on corneal thickness during and after storage. Curr Eye Res 10:1129-36 |
| Walkenbach, R J; Corwin, J G; Ye, G S (1991) Corneal function after storage in commercial eye bank media. Invest Ophthalmol Vis Sci 32:1551-7 |
| Walkenbach, R J; Ye, G S (1990) Muscarinic receptors and their regulation of cyclic GMP in corneal endothelial cells. Invest Ophthalmol Vis Sci 31:702-7 |
| Walkenbach, R J; Corwin, J G (1987) Corneal deturgescence during organ culture. Curr Eye Res 6:381-9 |
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