Abstract

This proposal involves the development and evaluation of new measurement and data-processing methods for clinical chemistry and biomedical studies. Approaches emphasized in the proposal are multipoint data-processing methods for kinetic analysis, adaptation and development of multiwavelength data-processing methods for multicomponent spectrochemical analysis in the presence of unknown interferences, adaptation of imaging detectors for multiwavelength fluorescence analysis, and simultaneous multielement analysis based on continuous-source atomic absorption spectroscopy. Goals of the kinetic studies are to develop data-processing methods that are applicable to noninteger reaction orders and will provide the same advantages of extended linear ranges, improved sensitivity, and markedly reduced errors that have characterized applications to first-order and Michaelis-Menten kinetics. Goals of the absorption and fluorescence spectroscopic studies are to provide quantitative approaches that will yield high quality results for multiple components in the """"""""dirty"""""""" matrices that are characteristic of biological samples. Goals of the atomic-absorption project are to provide the same type of multielement capability for atomic absorption that is common for atomic emission while retaining most of the simplicity that has contributed to the popularity of atomic absorption methods. Model reactions and chemical problems to be used in the development and evaluation of these approaches will be selected not only for their suitability for these studies but also for their significance to clinical chemistry and other biomedical areas.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM013326-23
Application #
3268479
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1978-12-01
Project End
1991-07-31
Budget Start
1990-07-01
Budget End
1991-07-31
Support Year
23
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Pardue, H L (1994) Systematic top-down approach to clinical chemistry. Clin Chim Acta 225:S13-33
Li, J; Pardue, H L (1994) Predictive steady-state chromatography. 1. Algorithms for leading and trailing edges of resolved and unresolved peaks in liquid chromatography. Anal Chem 66:3765-72
Pardue, H L; Truchaud, A; Osawa, K et al. (1994) International Federation of Clinical Chemistry (IFCC): systematic top-down approach to clinical chemistry. Ann Biol Clin (Paris) 52:311-20
Chen, E T; Pardue, H L (1994) Kinetic method for the quantitative resolution of structural isomers based on the catalytic properties of beta-cyclodextrin. Anal Chem 66:3318-22
Li, J; Pardue, H L (1993) Development and evaluation of an error-compensating predictive data-processing method for liquid chromatography. Anal Chem 65:1980-7
Uhegbu, C E; Lim, K B; Pardue, H L (1993) Initial studies of a new approach to the design and use of enzyme-based reactor/sensor systems: amperometric system for glucose. Anal Chem 65:2443-51
Laios, I D; Pardue, H L (1993) Kinetic study of the reaction of acetoacetate with glycine and sodium nitroprusside. Anal Chem 65:1903-9
Lim, K B; Pardue, H L (1993) Error-compensating kinetic method for enzymatic determination of DNAs. Clin Chem 39:1850-6
Chen, E T; Pardue, H L (1993) Analytical applications of catalytic properties of modified cyclodextrins. Anal Chem 65:2563-7
Hsiech, C; Pardue, H L (1993) Improved data-processing method for atomic absorption spectroscopy with electrothermal atomization. Anal Chem 65:1809-13

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