The broad goals of this project are to develop and evaluate improved analytical methods applicable to chemical analyses involved in understanding, diagnosing and treatment of illness. All analytical methods can be grouped into two general categories, namely kinetic-based and equilibrium-based management. This proposal focuses on kinetic- based methods which usually are less reliable than equilibrium-based methods. Specific goals of this study are to improve performance characteristics such as error coefficients, linear ranges, sensitivities, and ruggedness of kinetic-based analytical methodologies used in clinical and biological chemistry by factors of 10- to 100-fold relative to methods in current use. To accomplish these goals we plan to adapt a predictive kinetic method developed in earlier phases of this project to transient responses of analytical systems such as biosensors, ion-selective electrodes, selected types of immunoassays. and reactions of DNA. Evaluations of this data-processing approach relative to more conventional kinetic(rate) methods for homogeneous chemical reactions have yielded 10- to 100-fold improvements in error cooefficients for inorganic, organic, and enzymic reactions, 30-fold extensions of linear ranges for substrates of enzymatic reactions, and similar imrovement in sensitivities and ruggedness. There is reason to believe that this data-processing approach can yield similar improvements for analytical methodologies based on other types of chemical and physico-chemical systems that are important in clinical and biological chemistry.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM013326-27
Application #
2168645
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1978-12-01
Project End
1996-07-31
Budget Start
1994-08-01
Budget End
1996-07-31
Support Year
27
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Purdue University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Pardue, H L (1994) Systematic top-down approach to clinical chemistry. Clin Chim Acta 225:S13-33
Li, J; Pardue, H L (1994) Predictive steady-state chromatography. 1. Algorithms for leading and trailing edges of resolved and unresolved peaks in liquid chromatography. Anal Chem 66:3765-72
Pardue, H L; Truchaud, A; Osawa, K et al. (1994) International Federation of Clinical Chemistry (IFCC): systematic top-down approach to clinical chemistry. Ann Biol Clin (Paris) 52:311-20
Chen, E T; Pardue, H L (1994) Kinetic method for the quantitative resolution of structural isomers based on the catalytic properties of beta-cyclodextrin. Anal Chem 66:3318-22
Hsiech, C; Pardue, H L (1993) Improved data-processing method for atomic absorption spectroscopy with electrothermal atomization. Anal Chem 65:1809-13
Li, J; Pardue, H L (1993) Development and evaluation of an error-compensating predictive data-processing method for liquid chromatography. Anal Chem 65:1980-7
Uhegbu, C E; Lim, K B; Pardue, H L (1993) Initial studies of a new approach to the design and use of enzyme-based reactor/sensor systems: amperometric system for glucose. Anal Chem 65:2443-51
Laios, I D; Pardue, H L (1993) Kinetic study of the reaction of acetoacetate with glycine and sodium nitroprusside. Anal Chem 65:1903-9
Lim, K B; Pardue, H L (1993) Error-compensating kinetic method for enzymatic determination of DNAs. Clin Chem 39:1850-6
Chen, E T; Pardue, H L (1993) Analytical applications of catalytic properties of modified cyclodextrins. Anal Chem 65:2563-7

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