Vitamin B12, in the form of its coenzyme, is an obligatory cofactor in a series of twelve enzyme catalyzed reactions. The glutamate mutase, methyl- malonyl-CoA mutase and methylitaconate mutase enzymes are of outstanding interest because they catalyze carbon-skeleton rearrangements for which there have been no analogues in organic chemistry. The methylmalonyl-CoA to succinyl-CoA rearrangement is of crucial import in mammalian systems; it is the way the body converts propionate to succinate, leading into the main stream of biochemical metabolism. In this proposal, the mechanism by which these rearrangements occur will be explored using both model and enzymic systems. A new model for the methymalonyl-CoA system has been synthesized with an appended free radical trap to test for the occurrence of radical intermediates. This test will also be applied to the enzyme system. In addition, a new, synthetic malonate binding molecule will be used to study the reversibility of the model rearrangement. In order to facilitate the study of the function and structure of glutamate mutase, the gene will be cloned. Then, with adequate supplies of pure enzyme, mechanistic and structural studies will be initiated. Free radical and carbanion intermediates in the glutamate-methylaspartate rearrangement will be explored and a very successful Schiff base model will be developed. Surfactant-based models will be examined for their impact on our understanding of some unprecedented free radical migration reactions.
| Choi, G Y; Choi, S C; Galan, A et al. (1990) Vitamin B12S-promoted model rearrangement of methylmalonate to succinate is not a free radical reaction. Proc Natl Acad Sci U S A 87:3174-6 |
