Abstract

This research is designed to further elucidate the molecular mechanisms underlying the translational control of transcription termination in Escherichia coli K12 in general and to determine the genetic and biochemical regulatory mechanisms of isoleucine-valine biosynthesis specifically. In addition, fundamental knowledge about the utilization of individual tRNAs in translation and the interactions of translation machinery with RNA polymerase and termination factors will be gained. The work will employ biochemical, genetic, and physiological methodologies of molecular biology emphasizing recombinant DNA technology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM024330-08
Application #
3272208
Study Section
Biochemistry Study Section (BIO)
Project Start
1977-07-01
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
Schools of Medicine
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Parekh, B S; Hatfield, G W (1996) Transcriptional activation by protein-induced DNA bending: evidence for a DNA structural transmission model. Proc Natl Acad Sci U S A 93:1173-7
Pagel, J M; Winkelman, J W; Adams, C W et al. (1992) DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli. J Mol Biol 224:919-35
Pagel, J M; Hatfield, G W (1991) Integration host factor-mediated expression of the ilvGMEDA operon of Escherichia coli. J Biol Chem 266:1985-96
Winkelman, J W; Hatfield, G W (1990) Characterization of the integration host factor binding site in the ilvPG1 promoter region of the ilvGMEDA operon of Escherichia coli. J Biol Chem 265:10055-60
Gutman, G A; Hatfield, G W (1989) Nonrandom utilization of codon pairs in Escherichia coli. Proc Natl Acad Sci U S A 86:3699-703
Heck, J D; Hatfield, G W (1988) Valyl-tRNA synthetase gene of Escherichia coli K12. Primary structure and homology within a family of aminoacyl-TRNA synthetases. J Biol Chem 263:868-77
Wek, R C; Hatfield, G W (1988) Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli. J Mol Biol 203:643-63
Heck, J D; Hatfield, G W (1988) Valyl-tRNA synthetase gene of Escherichia coli K12. Molecular genetic characterization. J Biol Chem 263:857-67
Lawther, R P; Wek, R C; Lopes, J M et al. (1987) The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res 15:2137-55
Wek, R C; Sameshima, J H; Hatfield, G W (1987) Rho-dependent transcriptional polarity in the ilvGMEDA operon of wild-type Escherichia coli K12. J Biol Chem 262:15256-61

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