Abstract

Our investigations will emphasize the ways in which the molecular mechanisms that control the operons of the ilv regulon in Escherichia coli respond to physiological signals and how these operons coordinate their regulation. For example, we will study how the ilvBN and ilvGMEDA operons respond to carbon shifts during growth and what roles the attenuators play in these responses. We will also address a major question regarding attenuation which has not yet been seriously considered; that is, what is the importance of codon context in the leader polypeptides of attenuators. In this regard we have advanced an intriguing hypothesis for codon pair usage that may have broad implications for atenuation and protein synthesis. We further propose to determine the molecular mechanisms responsible for the regulation of the ilvYC gene and the valyl-tRNA synthetase gene, and the roles of the AHAS isozymes in the regulation of isoleucine-valine biosynthesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM024330-12
Application #
3272211
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1977-07-01
Project End
1990-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
12
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
Schools of Medicine
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Parekh, B S; Hatfield, G W (1996) Transcriptional activation by protein-induced DNA bending: evidence for a DNA structural transmission model. Proc Natl Acad Sci U S A 93:1173-7
Pagel, J M; Winkelman, J W; Adams, C W et al. (1992) DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli. J Mol Biol 224:919-35
Pagel, J M; Hatfield, G W (1991) Integration host factor-mediated expression of the ilvGMEDA operon of Escherichia coli. J Biol Chem 266:1985-96
Winkelman, J W; Hatfield, G W (1990) Characterization of the integration host factor binding site in the ilvPG1 promoter region of the ilvGMEDA operon of Escherichia coli. J Biol Chem 265:10055-60
Gutman, G A; Hatfield, G W (1989) Nonrandom utilization of codon pairs in Escherichia coli. Proc Natl Acad Sci U S A 86:3699-703
Heck, J D; Hatfield, G W (1988) Valyl-tRNA synthetase gene of Escherichia coli K12. Primary structure and homology within a family of aminoacyl-TRNA synthetases. J Biol Chem 263:868-77
Wek, R C; Hatfield, G W (1988) Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli. J Mol Biol 203:643-63
Heck, J D; Hatfield, G W (1988) Valyl-tRNA synthetase gene of Escherichia coli K12. Molecular genetic characterization. J Biol Chem 263:857-67
Lawther, R P; Wek, R C; Lopes, J M et al. (1987) The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12. Nucleic Acids Res 15:2137-55
Wek, R C; Sameshima, J H; Hatfield, G W (1987) Rho-dependent transcriptional polarity in the ilvGMEDA operon of wild-type Escherichia coli K12. J Biol Chem 262:15256-61

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