We propose to begin a molecular analysis of the sensory properties of the fungus, Phycomyces blakesleeanus. Several mutations have been identified which block signal transfer at specific steps in the sensory transduction pathway. We plan to design a vector system for manipulating Phycomyces genes and to work out a procedure for introducing such vectors into Phycomyces by transformation or by microinjection. We will attempt to identify the sensory genes from a genomic library by their ability to restore function when introduced into host strains carrying the appropriate mutations in the sensory pathway. We can then hope to begin a detailed molecular analysis of these genes and their products to explore how their interactions lay down the sensory network.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034605-03
Application #
3285909
Study Section
Genetics Study Section (GEN)
Project Start
1985-04-01
Project End
1989-03-31
Budget Start
1987-04-01
Budget End
1989-03-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92037