This research proposal has two objectives: 1) To study the packaging and arrangement of SV40 DNA in virus particles as a model for the higher order organization of cell chromatin; and 2) to study the relationship between the structures and biological activities of SV40 nucleoprotein complexes. Preliminary studies using psoralen derivatives to cross-link SV40 DNA in vivo or in situ in the virus particle have detected novel DNA-protein interactions of DNA in the virus particle and in SV40 replicaton complexes. Experiments are proposed to extend these analyses.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM036800-01
Application #
3291266
Study Section
Virology Study Section (VR)
Project Start
1985-09-01
Project End
1988-03-31
Budget Start
1985-09-01
Budget End
1986-03-31
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Mount Sinai School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029