This research proposal has two objectives: 1) To study the packaging and arrangement of SV40 DNA in virus particles as a model for the higher order organization of cell chromatin; and 2) to study the relationship between the structures and biological activities of SV40 nucleoprotein complexes. Preliminary studies using psoralen derivatives to cross-link SV40 DNA in vivo or in situ in the virus particle have detected novel DNA-protein interactions of DNA in the virus particle and in SV40 replicaton complexes. Experiments are proposed to extend these analyses.
| Wong, M L; Hsu, M T (1988) Psoralen-cross-linking study of the organization of intracellular adenovirus nucleoprotein complexes. J Virol 62:1227-34 |
| Hsu, M T; Parvin, J D; Gupta, S et al. (1987) Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. Proc Natl Acad Sci U S A 84:8140-4 |
