Abstract

Estrogen given to a properly primed mammal is a trigger for embryos to implant in the uterus and continue development. Data collected during the previous grant period, together with reports in the literature, suggest that estradiol may act indirectly via the uterine stroma to promote cleavage of heparan sulfate proteoglycans (HSPG) from the epithelial basolateral surface. This leads to a rounding of the epithelial cells and turnover of their anionic surface to the blastocyst. To test this idea we will (1) characterize the changes in the dominant anionic glycoconjugates on the epithelial surface during implantation, (2) examine the changes during implantation in stromal cell secretions, particularly lytic enzymes, (3) determine the influence of stromal secretions on epithelial ultrastructure and surface anionic glycoconjugates in epithelial-stromal combinations in culture, and (4) identify those epithelial surface anions that bind to and/or block adhesive sites on the blastocyst surface in culture.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD019530-05
Application #
3316842
Study Section
Reproductive Endocrinology Study Section (REN)
Project Start
1985-07-01
Project End
1994-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
5
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Oregon State University
Department
Type
Schools of Arts and Sciences
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339

  Publications

Chai, L; Morris, J E (1999) Heparan sulfate in the inner limiting membrane of embryonic chicken retina binds basic fibroblast growth factor to promote axonal outgrowth. Exp Neurol 160:175-85
Potter, S W; Gaza, G; Morris, J E (1996) Estradiol induces E-cadherin degradation in mouse uterine epithelium during the estrous cycle and early pregnancy. J Cell Physiol 169:1-14
Doss, R P; Potter, S W; Soeldner, A H et al. (1995) Adhesion of germlings of Botrytis cinerea. Appl Environ Microbiol 61:260-5
Chai, L; Morris, J E (1994) Distribution of heparan sulfate proteoglycans in embryonic chicken neural retina and isolated inner limiting membrane. Curr Eye Res 13:669-77
Morris, J E; Gaza, G; Potter, S W (1994) Specific stimulation of basal lamina heparan sulfate proteoglycan in mouse uterine epithelium by Matrigel and by transforming growth factor-beta 1. In Vitro Cell Dev Biol Anim 30A:120-8
Astrahantseff, K N; Morris, J E (1994) Estradiol-17 beta stimulates proliferation of uterine epithelial cells cultured with stromal cells but not cultured separately. In Vitro Cell Dev Biol Anim 30A:769-76
Morris, J E (1993) Proteoglycans and the modulation of cell adhesion by steric exclusion. Dev Dyn 196:246-51
Potter, S W; Morris, J E (1992) Changes in histochemical distribution of cell surface heparan sulfate proteoglycan in mouse uterus during the estrous cycle and early pregnancy. Anat Rec 234:383-90
Plytycz, B; Potter, S W; Cohen, N et al. (1988) In vitro fusion of newt macrophages. J Exp Zool 246:319-23
Morris, J E; Potter, S W; Gaza-Bulseco, G (1988) Estradiol induces an accumulation of free heparan sulfate glycosaminoglycan chains in uterine epithelium. Endocrinology 122:242-53

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