Abstract

This application will try to achieve the long term culture of type A spermatogonia.
Three specific aims are proposed: 1) to determine the basement membrane components that are optimal for allowing long-term (longer than 1 week) survival of type A spermatogonia in culture; 2) to test the hypothesis that there is synergism between growth factors and basement membrane components in promoting long-term survival of spermatogonia; and 3) to develop immortalized cell lines of type A spermatogonia that will propagate in culture without losing their original properties.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD036483-05
Application #
6521089
Study Section
Special Emphasis Panel (ZHD1-MRG-C (04))
Program Officer
Tasca, Richard J
Project Start
1998-04-01
Project End
2003-03-31
Budget Start
2002-04-01
Budget End
2003-03-31
Support Year
5
Fiscal Year
2002
Total Cost
$234,517
Indirect Cost

  Institution

Name
Georgetown University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057

  Publications

Braydich-Stolle, Laura; Kostereva, Natalia; Dym, Martin et al. (2007) Role of Src family kinases and N-Myc in spermatogonial stem cell proliferation. Dev Biol 304:34-45
Pang, Alan L Y; Johnson, Warren; Ravindranath, Neelakanta et al. (2006) Expression profiling of purified male germ cells: stage-specific expression patterns related to meiosis and postmeiotic development. Physiol Genomics 24:75-85
Vong, Queenie P; Li, Yunmin; Lau, Yun-Fai Chris et al. (2006) Structural characterization and expression studies of Dby and its homologs in the mouse. J Androl 27:653-61
Hofmann, Marie-Claude; Braydich-Stolle, Laura; Dettin, Luis et al. (2005) Immortalization of mouse germ line stem cells. Stem Cells 23:200-10
Hofmann, Marie-Claude; Braydich-Stolle, Laura; Dym, Martin (2005) Isolation of male germ-line stem cells; influence of GDNF. Dev Biol 279:114-24
Hofmann, Marie-Claude; Van Der Wee, Katherine S; Dargart, Jamie L et al. (2003) Establishment and characterization of neonatal mouse sertoli cell lines. J Androl 24:120-30
Pang, Alan L Y; Taylor, H Claire; Johnson, Warren et al. (2003) Identification of differentially expressed genes in mouse spermatogenesis. J Androl 24:899-911
Feng, Li-Xin; Chen, Yali; Dettin, Luis et al. (2002) Generation and in vitro differentiation of a spermatogonial cell line. Science 297:392-5
van der Wee, K S; Johnson, E W; Dirami, G et al. (2001) Immunomagnetic isolation and long-term culture of mouse type A spermatogonia. J Androl 22:696-704