The proposal concerns the formation of left-right asymmetry in heart development. How this asymmetry arises is a basic question in developmental biology, which also has important clinical implications. Dr. Yost has published several articles in the field, mainly with an emphasis on the relatively late role of extracellular matrix, specifically, proteoglycans, in regulating left-right asymmetry. The experiments set out in this proposal will extend on these earlier studies, by focusing more on the earlier embryonic events that regulate left-right asymmetry, beginning with the potential role of maternal components, and progressing into gastrulation stages.
| Tsai, I-Chun; Amack, Jeffrey D; Gao, Zhong-Hua et al. (2007) A Wnt-CKIvarepsilon-Rap1 pathway regulates gastrulation by modulating SIPA1L1, a Rap GTPase activating protein. Dev Cell 12:335-47 |
| Luo, Wen; Peterson, Annita; Garcia, Benjamin A et al. (2007) Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex. EMBO J 26:1511-21 |
| Bisgrove, Brent W; Morelli, Susan H; Yost, H Joseph (2003) Genetics of human laterality disorders: insights from vertebrate model systems. Annu Rev Genomics Hum Genet 4:1-32 |
| Branford, William W; Yost, H Joseph (2002) Lefty-dependent inhibition of Nodal- and Wnt-responsive organizer gene expression is essential for normal gastrulation. Curr Biol 12:2136-41 |
| Yost, H J (2000) Specification of cardiac mesenchyme and heart morphogenesis in vitro. Methods Mol Biol 136:39-43 |
