The overall purpose of this application is to refine existing techniques and to develop new biochemical models, methods, and experimental techniques for autoradiographic and magnetic resonance imaging of cerebral blood flow and metabolism and their interrelationships. The development of a very high performance, low cost imaging system for quantitative analysis and three dimensional processing and display of autoradiographic images will be completed. The system uses newly-developed memory-intensive, lookup table-based algorithms to achieve very rapid effective processing speeds on personal computers. The system will be used for three dimensional quantitative analysis of autoradiograms of cerebral blood flow and metabolism tracers in animals. Double and triple label autoradiographic techniques will be developed for concurrent and sequential tracer studies. Using these techniques, the kinetics of various metabolic tracers will be compared. Results of these studies will be used to evaluate existing kinetic models, and to assess the significance and limitations of their basic assumptions. From this base, new methods for estimatiOn of cerebral glycolysis and oxidation will be developed. The metabolic studies will then be combined with studies of blood flow under normal and abnormal conditions. Based on autoradiographic studies, corresponding magnetic resonance (MR) imaging and spectroscopic studies will be performed In humans to evaluate the relationships between cerebral blood flow (deoxyhemoglobin-shift imaging) and cerebral metabolism (lactate spectroscopy). The basic understanding of cerebral metabolism and blood flow provided by these studies will promote greater understanding of the function of the brain in normal and pathological conditions in animal models (autoradiography) and in humans (MR).

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS026657-05A2
Application #
2266035
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1988-12-01
Project End
1997-05-31
Budget Start
1994-06-01
Budget End
1995-05-31
Support Year
5
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
Trujillo, N P; Pratt, J P; Talusani, S et al. (1997) DTPA aerosol in ventilation/perfusion scintigraphy for diagnosing pulmonary embolism. J Nucl Med 38:1781-3
Lear, J L; Pratt, J P; Trujillo, N (1996) Redundant array of independent disks: practical on-line archiving of nuclear medicine image data. J Digit Imaging 9:37-8
Lear, J L; Pratt, J P; Mallet, J et al. (1994) User-definable bull's-eye database analysis. Eur J Nucl Med 21:154-7
Pratt, J P; Lear, J L (1993) Increased single-photon emission computed tomography image processing speed achieved in personal computers with memory-intensive algorithms. J Digit Imaging 6:205-12
Lear, J L; Kasliwal, R; Duryea, R A (1992) Effects of variations in the duration of diffusible-tracer infusions on calculated values of global and local cerebral blood flow. Metab Brain Dis 7:197-210
Lear, J L; Ackerman, R F (1992) Comparison of regional blood-brain transport kinetics between glucose and fluorodeoxyglucose. J Nucl Med 33:1819-24
Lear, J L (1991) Relationship between myocardial clearance rates of carbon-11-acetate-derived radiolabel and oxidative metabolism: physiologic basis and clinical significance. J Nucl Med 32:1957-60
Lear, J L; Kasliwal, R K (1991) Autoradiographic measurement of cerebral lactate transport rate constants in normal and activated conditions. J Cereb Blood Flow Metab 11:576-80
Lear, J L; Kasliwal, R K; Feyerabend, A J et al. (1991) Improved tumor imaging with radiolabeled monoclonal antibodies by plasma clearance of unbound antibody with anti-antibody column. Radiology 179:509-12
Lear, J L; Ackermann, R F (1990) Quantification of patterns of regional cardiac metabolism. Radiology 176:659-64

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