Abstract

The long-term objective is to understand neural control of hormone secretion and its impact on reproductive physiology and behavior using the marine mollusk Aplysia californica as an animal model. Because of their simple nervous system and large neurons, Aplysia have been widely used for biophysical, cellular and molecular investigations of the neuroendocrine cells (called bag cells) that control ovulation and egg-laying behavior. However, there has been little work on the processes that regulate hormone secretion from these cells. The proposed work will use bag cells and their secretion of egg-laying hormone (ELH) to identify cellular mechanisms that regulate dynamic changes in hormone secretion.
The specific aims are to determine: whether patterns of bag-cell electrical afterdischarge and ELH secretion are similar between in vivo and in vitro preparations; and to determine the role of the diacylglycerol (DAG), calcium and cAMP in regulating ELH secretion.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS033548-03
Application #
2037868
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Program Officer
Streicher, Eugene
Project Start
1994-12-01
Project End
1998-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Physiology
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Lee, Wenjau; Wayne, Nancy L (2004) Secretion of locally synthesized neurohormone from neurites of peptidergic neurons. J Neurochem 88:532-7
Wayne, Nancy L; Lee, Wenjau; Michel, Stephan et al. (2004) Post-afterdischarge depolarization does not stimulate prolonged neurohormone secretion but is required for activity-dependent stimulation of neurohormone biosynthesis from peptidergic neurons. Endocrinology 145:1678-84
Wayne, Nancy L; Michel, Stephan (2004) Synaptic stimulation of Aplysia peptidergic neurons can activate hormone secretion in the absence of an afterdischarge. Neurosci Lett 362:249-52
Wayne, Nancy L; Lee, Wenjau; Michel, Stephan et al. (2004) Activity-dependent regulation of neurohormone synthesis and its impact on reproductive behavior in aplysia. Biol Reprod 70:277-81
Dyer, John R; Michel, Stephan; Lee, Wenjau et al. (2003) An activity-dependent switch to cap-independent translation triggered by eIF4E dephosphorylation. Nat Neurosci 6:219-20
Lee, Wenjau; Jones, Andrea M; Ono, Joyce K et al. (2002) Regional differences in processing of locally translated prohormone in peptidergic neurons of Aplysia californica. J Neurochem 83:1423-30
Michel, Stephan; Wayne, Nancy L (2002) Neurohormone secretion persists after post-afterdischarge membrane depolarization and cytosolic calcium elevation in peptidergic neurons in intact nervous tissue. J Neurosci 22:9063-9
Wayne, N L; Lee, W; Kim, Y J (1999) Persistent activation of calcium-activated and calcium-independent protein kinase C in response to electrical afterdischarge from peptidergic neurons of aplysia. Brain Res 834:211-3
Wayne, N L; Kim, Y J; Yong-Montenegro, R J (1998) Seasonal fluctuations in the secretory response of neuroendocrine cells of Aplysia californica to inhibitors of protein kinase A and protein kinase C. Gen Comp Endocrinol 109:356-65
Wayne, N L; Kim, J; Lee, E (1998) Prolonged hormone secretion from neuroendocrine cells of Aplysia is independent of extracellular calcium. J Neuroendocrinol 10:529-37

Showing the most recent 10 out of 12 publications