Abstract

description): The objective of this project is the cloning of the gene encoding mitochondrial carnitine/acylcarnitine translocase. The new set of Specific Aims include: 1) cloning and sequencing of the human CAC, cDNA and structural gene, 2) sequencing of CAC deficiency mutant genes, 3) examination of the expression of the mutant genes in mutant fibroblast cell lines, 4) creation of mutants in a yeast gene, yOR100c, that they believe to be yeast CAC and assessment of its genetic function, and 5) assaying CAC activity in mammalian cells in which mutant and wild-type CAC has been introduced.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Small Research Grants (R03)
Project #
1R03HD035685-01A1
Application #
2555865
Study Section
Pediatrics Subcommittee (CHHD)
Project Start
1998-03-01
Project End
2000-02-29
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199