The proposal intends to extend and refine the investigators' previous observations on T cell turnover in HIV infection using a stable isotope-mass spectrometric (MS) method in which cellular DNA is labelled with 2-H glucose. Studies will be performed in seronegative controls and in seropositive subjects before and after antiretroviral therapy. The goals of the project are: 1) to attempt to simplify the method with shorter time of administration and/or oral administration, 2) utilize a different MS analytical process to reduce cost and 3) use the method to follow additional subpopulations of cells, including antigen specific and V-beta subpopulations.
| Busch, Robert; Kim, Yoo-Kyeong; Neese, Richard A et al. (2006) Measurement of protein turnover rates by heavy water labeling of nonessential amino acids. Biochim Biophys Acta 1760:730-44 |
| Hellerstein, Marc K; Hoh, Rebecca A; Hanley, Mary Beth et al. (2003) Subpopulations of long-lived and short-lived T cells in advanced HIV-1 infection. J Clin Invest 112:956-66 |
| Hellerstein, M K (2001) Pathophysiology of body composition and metabolic abnormalities in HIV-infection: therapeutic implications. Int J Sport Nutr Exerc Metab 11 Suppl:S105-10 |
| Neese, R A; Siler, S Q; Cesar, D et al. (2001) Advances in the stable isotope-mass spectrometric measurement of DNA synthesis and cell proliferation. Anal Biochem 298:189-95 |
| Hellerstein, M K (1999) Measurement of T-cell kinetics: recent methodologic advances. Immunol Today 20:438-41 |
