The proposal intends to extend and refine the investigators' previous observations on T cell turnover in HIV infection using a stable isotope-mass spectrometric (MS) method in which cellular DNA is labelled with 2-H glucose. Studies will be performed in seronegative controls and in seropositive subjects before and after antiretroviral therapy. The goals of the project are: 1) to attempt to simplify the method with shorter time of administration and/or oral administration, 2) utilize a different MS analytical process to reduce cost and 3) use the method to follow additional subpopulations of cells, including antigen specific and V-beta subpopulations.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21AI044767-02
Application #
2887957
Study Section
Special Emphasis Panel (ZRG5-AARR-2 (03))
Program Officer
Finerty, John F
Project Start
1998-09-30
Project End
2001-03-31
Budget Start
1999-09-30
Budget End
2001-03-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Nutrition
Type
Schools of Earth Sciences/Natur
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Busch, Robert; Kim, Yoo-Kyeong; Neese, Richard A et al. (2006) Measurement of protein turnover rates by heavy water labeling of nonessential amino acids. Biochim Biophys Acta 1760:730-44
Hellerstein, Marc K; Hoh, Rebecca A; Hanley, Mary Beth et al. (2003) Subpopulations of long-lived and short-lived T cells in advanced HIV-1 infection. J Clin Invest 112:956-66
Hellerstein, M K (2001) Pathophysiology of body composition and metabolic abnormalities in HIV-infection: therapeutic implications. Int J Sport Nutr Exerc Metab 11 Suppl:S105-10
Neese, R A; Siler, S Q; Cesar, D et al. (2001) Advances in the stable isotope-mass spectrometric measurement of DNA synthesis and cell proliferation. Anal Biochem 298:189-95
Hellerstein, M K (1999) Measurement of T-cell kinetics: recent methodologic advances. Immunol Today 20:438-41