The major goal is to identify schistosome proteins important in immunity and development through the use of recombinant DNA technology. To accomplish this goal we will take advantage of the fact that in concomitant or attenuated parasite immunity, there are species of antibody molecules that recognize determinants on the surface of the schistosomule that mediate a schistosomicidal response. These antibodies provide appropriate reagents for isolating relevant antigens, when used in conjunction with recombinant DNA technology. By isolating the DNA encoding an important antigen, we can sequence the gene and deduce the amino acid sequence of the antigen, produce reasonable quantities of the antigen in bacteria for vaccine testing, create a synthetic recombinant vaccinia virus encoding the antigen, or create a modified antigen that may function even better than the native one in producing immunity. In addition, the availability of purified protein antigens will allow us to develop monospecific sera which, together with the DNA encoding the antigens will be useful in exploring the control and expression of these proteins during development. By assessing the efficacy of identified antigens in protection experiments we will be able to identify those that are relevant. Once relevant antigens are identified, we will assess recombinant vaccinia virus as a mode of antigen presentation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI018867-05
Application #
3444586
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1983-04-01
Project End
1989-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost
Name
State University of New York at Buffalo
Department
Type
School of Medicine & Dentistry
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Corstjens, Paul L A M; De Dood, Claudia J; Kornelis, Dieuwke et al. (2014) Tools for diagnosis, monitoring and screening of Schistosoma infections utilizing lateral-flow based assays and upconverting phosphor labels. Parasitology 141:1841-55
Mohamed, M R; Shalaby, K A; LoVerde, P T et al. (2008) Cloning and characterization of a cDNA fragment encoding a Schistosoma mansoni actin-binding protein (Smfilamin). Parasitol Res 102:1035-42
Deng, Jiusheng; Gold, Daniel; LoVerde, Philip T et al. (2007) Mapping of the complement C9 binding domain in paramyosin of the blood fluke Schistosoma mansoni. Int J Parasitol 37:67-75
Cook, Rosemary M; Carvalho-Queiroz, Claudia; Wilding, Gregory et al. (2004) Nucleic acid vaccination with Schistosoma mansoni antioxidant enzyme cytosolic superoxide dismutase and the structural protein filamin confers protection against the adult worm stage. Infect Immun 72:6112-24
LoVerde, Philip T; Carvalho-Queiroz, Claudia; Cook, Rosemary (2004) Vaccination with antioxidant enzymes confers protective immunity against challenge infection with Schistosoma mansoni. Mem Inst Oswaldo Cruz 99:37-43
Deng, Jiusheng; Gold, Daniel; LoVerde, Philip T et al. (2003) Inhibition of the complement membrane attack complex by Schistosoma mansoni paramyosin. Infect Immun 71:6402-10
Shalaby, Kamal A; Yin, Lei; Thakur, Arvind et al. (2003) Protection against Schistosoma mansoni utilizing DNA vaccination with genes encoding Cu/Zn cytosolic superoxide dismutase, signal peptide-containing superoxide dismutase and glutathione peroxidase enzymes. Vaccine 22:130-6
El-Dabaa, E; Mei, H; El-Sayed, A et al. (1998) Cloning and characterization of Schistosoma mansoni fructose-1,6-bisphosphate aldolase isoenzyme. J Parasitol 84:954-60
Mohamed, M M; Shalaby, K A; LoVerde, P T et al. (1998) Characterization of Sm20.8, a member of a family of schistosome tegumental antigens. Mol Biochem Parasitol 96:15-25
Mei, H; LoVerde, P T (1997) Schistosoma mansoni: the developmental regulation and immunolocalization of antioxidant enzymes. Exp Parasitol 86:69-78

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