Schistosomiasis is a disease of man and animals caused by parasitic trematodes of the genus Schistosoma. This proposal seeks to isolate, characterize, and to test experimentally potential common target antigens or antigenic determinants from various trematodes including Fasciola, Schistosoma, and Paragonimus as to their protective effect primarily to Schistosoma, and to a lesser extent, Fasciola. Monoclonal anitbodies will be developed for the identification and isolation of defined immunity antigens. Emphasis will be on the development of monoclonal antibodies against different epitopes of trematode common surface antigens. The antibodies will then be used to isolate the antigens of interest. The purified antigens will then be characterized biochemically and their protective effects will be ascertained.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI020974-03
Application #
3444683
Study Section
(SSS)
Project Start
1984-04-01
Project End
1987-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Puerto Rico Rio Piedras
Department
Type
Schools of Arts and Sciences
DUNS #
City
San Juan
State
PR
Country
United States
Zip Code
00931
Santiago, N; Hillyer, G V (1988) Antibody profiles by EITB and ELISA of cattle and sheep infected with Fasciola hepatica. J Parasitol 74:810-8
Hillyer, G V; Taylor, D W (1988) Immunoprecipitation of Fasciola hepatica mRNA in vitro translation products using infection and hyperimmune sera. Am J Trop Med Hyg 38:547-52
Hillyer, G V; Soler de Galanes, M; Garcia Rosa, M I et al. (1988) Acquired immunity in schistosomiasis with purified Fasciola hepatica cross-reactive antigens. Vet Parasitol 29:265-80
Hillyer, G V; Garcia Rosa, M I; Alicea, H et al. (1988) Successful vaccination against murine Schistosoma mansoni infection with a purified 12 Kd Fasciola hepatica cross-reactive antigen. Am J Trop Med Hyg 38:103-10
Haroun, E M; Hillyer, G V (1988) Cross-resistance between Schistosoma mansoni and Fasciola hepatica in sheep. J Parasitol 74:790-5
Hillyer, G V; Haroun, E T; Hernandez, A et al. (1987) Acquired resistance to Fasciola hepatica in cattle using a purified adult worm antigen. Am J Trop Med Hyg 37:363-9
Hillyer, G V; Richardson, B A; Butterworth, A E (1987) Failure of anti-Fasciola antisera to induce antibody-dependent, eosinophil-mediated killing of Schistosoma mansoni schistosomula. J Parasitol 73:774-7
Hillyer, G V (1987) Heterologous resistance in schistosomiasis. Mem Inst Oswaldo Cruz 82 Suppl 4:171-4
Haroun, E T; Hillyer, G V (1986) Resistance to fascioliasis--a review. Vet Parasitol 20:63-93
Hillyer, G V (1985) Induction of immunity in mice to Fasciola hepatica with a Fasciola/Schistosoma cross-reactive defined immunity antigen. Am J Trop Med Hyg 34:1127-31