This project used cultured rat olfactory receptor cells to elucidate mechanism(s) of olfactory transduction and/or adaptation. These cells respond to a variety of odorants with rapid, dose-dependent, transient increases in intracellular cAMP. The first goal is to determine what classes of odorants change levels of cAMP, measured by radioimmunoassay, and to study the regulation of adenylate cyclase and phosphodiesterase activities during exposure to these odorants. The second goal is to determine if the same, or possibly other, classes of odorants regulate phsophoinositide turnover by comparison of PI and IP pools in radiolabelled cultures. Two-dimensional gel chromatography will be used to identify any proteins that are potentially phosphorylated upon odor stimulation. The third goal is to investigate the role of odorant binding protein in olfaction by determining the effect of OBP on the dose-response curves of odorant-induced changes in cAMP. These experiments will use recombinant rat OBP. A final series of experiments will determine if co-culturing primary olfactory receptor neurons with olfactory bulb alters the ability of odors to induct changes in levels of cAMP and, potentially, PI turnover and protein phosphorylation.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29DC000872-02
Application #
3461743
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1990-07-01
Project End
1995-06-30
Budget Start
1991-07-01
Budget End
1992-06-30
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218