Abstract

The GABAergic system may play a significant role in the pathogenesis as well as in the pharmacological control of epilepsy and other brain diseases. Concentrations and rates of synthesis of GABA may reflect the activity of the GABAergic system. The goal of the proposed research is to develop quantitative single volume and multivolume 1H NMR methods for measuring brain gamma amino butyric acid (GABA) concentration and rates of synthesis in human brain. The methods will be applied in studies of human subjects to determine the precision of the measurement, the accuracy of the resonance assignment, and to measure the relaxation properties needed for GABA absolute quantitation. An animal model will be used to validate the NMR method by comparison with conventional chemical assay. The development of 1H NMR methods for measuring GABA will provide an important new tool for studying the human GABAergic system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29NS032126-04
Application #
2416332
Study Section
Diagnostic Radiology Study Section (RNM)
Program Officer
Jacobs, Margaret
Project Start
1994-05-01
Project End
1999-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Goddard, Andrew W; Mason, Graeme F; Appel, Michael et al. (2004) Impaired GABA neuronal response to acute benzodiazepine administration in panic disorder. Am J Psychiatry 161:2186-93
Hyder, Fahmeed; Kida, Ikuhiro; Behar, Kevin L et al. (2003) Dominant events that modulate mass transfer coefficient of oxygen in cerebral cortex. Adv Exp Med Biol 530:401-11
Shulman, Robert G; Hyder, Fahmeed; Rothman, Douglas L (2003) Cerebral metabolism and consciousness. C R Biol 326:253-73
Rothman, Douglas L; Behar, Kevin L; Hyder, Fahmeed et al. (2003) In vivo NMR studies of the glutamate neurotransmitter flux and neuroenergetics: implications for brain function. Annu Rev Physiol 65:401-27
Xu, Fuqiang; Liu, Nian; Kida, Ikuhiro et al. (2003) Odor maps of aldehydes and esters revealed by functional MRI in the glomerular layer of the mouse olfactory bulb. Proc Natl Acad Sci U S A 100:11029-34
Shulman, Robert G; Hyder, Fahmeed; Rothman, Douglas L (2002) Biophysical basis of brain activity: implications for neuroimaging. Q Rev Biophys 35:287-325
Smith, Arien J; Blumenfeld, Hal; Behar, Kevin L et al. (2002) Cerebral energetics and spiking frequency: the neurophysiological basis of fMRI. Proc Natl Acad Sci U S A 99:10765-70
Hyder, Fahmeed; Rothman, Douglas L; Shulman, Robert G (2002) Total neuroenergetics support localized brain activity: implications for the interpretation of fMRI. Proc Natl Acad Sci U S A 99:10771-6
Behar, K L; Rothman, D L (2001) In vivo nuclear magnetic resonance studies of glutamate-gamma-aminobutyric acid-glutamine cycling in rodent and human cortex: the central role of glutamine. J Nutr 131:2498S-504S; discussion 2523S-4S
Hyder, F; Kida, I; Behar, K L et al. (2001) Quantitative functional imaging of the brain: towards mapping neuronal activity by BOLD fMRI. NMR Biomed 14:413-31

Showing the most recent 10 out of 55 publications