Abstract

Specific Aims: Drs. Jonsson and Linial propose collaborative experiments to analyze the RNA: protein interactions leading to genomic RNA encapsidation for two different classes of viruses, hantaviruses and retroviruses. The Jonsson lab brings to this proposal expertise in hantavirology and biochemistry and chemistry of RNA-protein interactions. The Lineal lab provides the expertise in retrovirology and molecular genetic analysis of RNA-protein interactions using yeast. For retroviruses, we have chosen the avian or alpharetroviruses, Rous sarcoma virus (RSV). Work on the packaging signal of these viruses has pin pointed a small, contiguous RNA sequence that interacts specifically with residues in the nucleocapsid (NC ) domain of the viral structural protein precursor Gag. For the hantaviruses, we will use the Hantaan virus (HTNV). A putative packaging signal has been identified in the S- segment of the HTNV to interact with the nucleoprotein (N). The two specific aims of this pilot project are:
Aim 1). To identify the domain/amino acids in the HTNV N protein responsible for interaction with viral genomic and anti-genomic RNA using the yeast three-hybrid assay (YTHA), and Aim 2). To characterize the chemistry of the interaction of the RSV NC with its packaging signal, psi.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory Grants--Cooperative Agreements (U56)
Project #
1U56CA096286-01
Application #
6606801
Study Section
Special Emphasis Panel (ZCA1)
Project Start
2002-06-12
Project End
2007-05-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
New Mexico State University Las Cruces
Department
Type
DUNS #
City
Las Cruces
State
NM
Country
United States
Zip Code
88003

  Publications

Unguez, Graciela A (2013) Electric fish: new insights into conserved processes of adult tissue regeneration. J Exp Biol 216:2478-86
Thompson, Beti; O'Connell, Mary; Löest, Helena et al. (2013) Understanding and reducing obstacles in a collaboration between a minority institution and a cancer center. J Health Care Poor Underserved 24:1648-56
Voorhies, Wayne A Van (2012) Robust metabolic responses to varied carbon sources in natural and laboratory strains of Saccharomyces cerevisiae. PLoS One 7:e30053
Weber, Christopher M; Martindale, Mark Q; Tapscott, Stephen J et al. (2012) Activation of Pax7-positive cells in a non-contractile tissue contributes to regeneration of myogenic tissues in the electric fish S. macrurus. PLoS One 7:e36819
Icreverzi, Amalia; de la Cruz, Aida Flor; Van Voorhies, Wayne A et al. (2012) Drosophila cyclin D/Cdk4 regulates mitochondrial biogenesis and aging and sensitizes animals to hypoxic stress. Cell Cycle 11:554-68
Vermillion, Karl; Holguin, F Omar; Berhow, Mark A et al. (2011) Dinoxin B, a withanolide from Datura inoxia leaves with specific cytotoxic activities. J Nat Prod 74:267-71
Celotto, Alicia M; Chiu, Wai Kan; Van Voorhies, Wayne et al. (2011) Modes of metabolic compensation during mitochondrial disease using the Drosophila model of ATP6 dysfunction. PLoS One 6:e25823
Siamakpour-Reihani, Sharareh; Peterson, Tabitha A; Bradford, Andrew M et al. (2011) Grb7 binds to Hax-1 and undergoes an intramolecular domain association that offers a model for Grb7 regulation. J Mol Recognit 24:314-21
Pias, Sally; Peterson, Tabitha A; Johnson, Dennis L et al. (2010) The intertwining of structure and function: proposed helix-swapping of the SH2 domain of Grb7, a regulatory protein implicated in cancer progression and inflammation. Crit Rev Immunol 30:299-304
Coronado, Gloria D; O'Connell, Mary A; Anderson, Jennifer et al. (2010) Undergraduate cancer training program for underrepresented students: findings from a minority institution/cancer center partnership. J Cancer Educ 25:32-5

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