Molecular Genetic Analysis of Lymphocyte Function

Margulies, D H.

Abstract

The ongoing focus of the laboratory is to understand in detail the structure/function relationships of cell surface molecules involved in the immune response. In particular we have made significant progress over the past year in five areas: A. Using molecular engineering techniques to generate genes capable of encoding soluble murine class I major histocompatibility complex (MHC) antigens; B. Using the purified soluble antigens derived from these cells to analyze the biochemical and functional behavior of soluble H-2Dd; C. Analyzing the origin and function of proteins derived from alternatively spliced mRNAs of the class I antigens H-2Dd and H-2Kb; D. Analyzing the structure-function relationship of class I molecules by a detailed serological, biochemical and functional study of H-2Dd/H-2Ld chimeric proteins; E. Studying the function of in vitro class II/class I chimeric genes.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000394-03
• Application #: 3960584
• Support Year: 3
• Fiscal Year: 1986

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Morozov, Giora I; Zhao, Huaying; Mage, Michael G et al. (2016) Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing. Proc Natl Acad Sci U S A 113:E1006-15

Margulies, David H (2009) Home schooling of NK cells. Immunity 30:313-5

Hu, Jin-Shan; Plaksin, Daniel; Margulies, David H (2005) Backbone and side chain resonance assignmentsof a TRAV14-3 mouse T cell receptor domain. J Biomol NMR 31:271-2

Margulies, David H (2005) Monoclonal antibodies: producing magic bullets by somatic cell hybridization. J Immunol 174:2451-2

Candon, Sophie; McHugh, Rebecca S; Foucras, Gilles et al. (2004) Spontaneous organ-specific Th2-mediated autoimmunity in TCR transgenic mice. J Immunol 172:2917-24

Kuribayashi, Hideki; Wakabayashi, Ayako; Shimizu, Masumi et al. (2004) Resistance to viral infection by intraepithelial lymphocytes in HIV-1 P18-I10-specific T-cell receptor transgenic mice. Biochem Biophys Res Commun 316:356-63

Dam, Julie; Guan, Rongjin; Natarajan, Kannan et al. (2003) Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b). Nat Immunol 4:1213-22

Lukashev, Dmitriy E; Caldwell, Charles C; Chen, Pearl et al. (2003) A serine/threonine phosphorylation site in the ectodomain of a T cell receptor beta chain is required for activation by superantigen. J Recept Signal Transduct Res 23:33-52

Margulies, David H (2003) CD28, costimulator or agonist receptor? J Exp Med 197:949-53

Margulies, David H (2003) Molecular interactions: stiff or floppy (or somewhere in between?). Immunity 19:772-4