Abstract

The advance of genomics has led to the definition of thousands of human genes as well as the identification of a significant proportion of genes in other non-human organisms. We have coupled these advanced genomic resources with modern engineering and informatics capabilities to establish a cDNA microarray center laboratory. Microarrays allow for the simultaneous monitoring of gene expression changes relative to control in thousands of genes. It is the goal of this lab to aid intramural and extramural investigators in understanding how environmental agents may affect gene expression, and thus increase our knowledge of the mechanism of action of such agents. In the past year progress has been made to investigate the gene expression signatures associated with exposure to oxidant stressors (ie radiation, dioxin) and non-genotoxic carcinogens, such as peroxisome proliferators, phenobarbital, arsenic, and estrogen and oxidant stressors in defined human tissue culture models as well as in tissues from derived from animals exposed in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES023026-02
Application #
6432274
Study Section
(LMC)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Ferrucio, Bianca; Tiago, Manoela; Fannin, Richard D et al. (2017) Molecular effects of 1-naphthyl-methylcarbamate and solar radiation exposures on human melanocytes. Toxicol In Vitro 38:67-76
Cui, Yuxia; Huang, Qihong; Auman, James Todd et al. (2011) Genomic-derived markers for early detection of calcineurin inhibitor immunosuppressant-mediated nephrotoxicity. Toxicol Sci 124:23-34
Kienhuis, Anne S; van de Poll, Marcel C G; Wortelboer, Heleen et al. (2009) Parallelogram approach using rat-human in vitro and rat in vivo toxicogenomics predicts acetaminophen-induced hepatotoxicity in humans. Toxicol Sci 107:544-52
Chou, Jeff W; Zhou, Tong; Kaufmann, William K et al. (2007) Extracting gene expression patterns and identifying co-expressed genes from microarray data reveals biologically responsive processes. BMC Bioinformatics 8:427
Auman, J Todd; Chou, Jeff; Gerrish, Kevin et al. (2007) Identification of genes implicated in methapyrilene-induced hepatotoxicity by comparing differential gene expression in target and nontarget tissue. Environ Health Perspect 115:572-8
Auman, J Todd; Boorman, Gary A; Wilson, Ralph E et al. (2007) Heat map visualization of high-density clinical chemistry data. Physiol Genomics 31:352-6
Liu, Jie; Xie, Yaxiong; Ducharme, Danica M K et al. (2006) Global gene expression associated with hepatocarcinogenesis in adult male mice induced by in utero arsenic exposure. Environ Health Perspect 114:404-11
Liu, Jie; Xie, Yaxiong; Merrick, B Alex et al. (2006) Transplacental arsenic plus postnatal 12-O-teradecanoyl phorbol-13-acetate exposures associated with hepatocarcinogenesis induce similar aberrant gene expression patterns in male and female mouse liver. Toxicol Appl Pharmacol 213:216-23
Zhou, Tong; Chou, Jeff W; Simpson, Dennis A et al. (2006) Profiles of global gene expression in ionizing-radiation-damaged human diploid fibroblasts reveal synchronization behind the G1 checkpoint in a G0-like state of quiescence. Environ Health Perspect 114:553-9
Kim, Yongbaek; Ton, Thai-Vu; DeAngelo, Anthony B et al. (2006) Major carcinogenic pathways identified by gene expression analysis of peritoneal mesotheliomas following chemical treatment in F344 rats. Toxicol Appl Pharmacol 214:144-51

Showing the most recent 10 out of 57 publications