The past year has been very busy for the NIAMS Imaging Facility. Publications that include microscopy work carried out in the Light Imaging Section have been published in Blood, Immunology, Human Molecular Genetics and other high-quality scientific journals. These publications expand our knowledge of autoimmunity, muscle, and skin diseases. Researchers from 14 Sections, Laboratories, or Branches have used the Facility, 35 researchers have been trained to use one or more of the instruments and one presentation on the Bases of Microscopy & Ethical Imaging was offered to postdoctoral and postbac trainees. As every year, LIS staff have assisted Facility users to apply advanced microscopy techniques, to improve the quality of their images, to quantitate the images, and to prepare these images for publication. LIS staff have also evaluated new instruments that Facility users are interested in and worked on the replacement of out-of-date equipment. A Leica SP8 confocal microscope has replaced the Leica SP5 which dated from 2007. The SP8, besides producing high quality images is equipped with the Lightning software which offers super-resolution imaging. Its white light laser provides great flexibility in the choice of fluorophores to image. We have also recently replaced our workstation computer with a new, faster PC. The workstation drives our Incucytes and allows analysis of Incucyte data. It is also loaded with the LASX software that drives the Leica SP8 and allows analysis of the confocal images away from the instrument itself. It will also allow 3D image analysis with the Imaris software and post-acquisition deconvolution of confocal images. All our instruments have been in demand, including the Leica SP8 and the Zeiss 780 confocal microscopes, the Hamamatsu laser scanner, the Essen Zoom and S3 Incucytes, the Leica TIRF, and the Leica upright DM6000 and inverted DMI6000 fluorescence microscopes. The SP8 has been used in two-photon mode to collect Second Harmonic Generation images from muscle and teeth. There has been a sharp increase this year in the demand for slide scanning. More than 3,100 slides have been scanned in a year for scientists in 8 Laboratories/Branches working on arthritis, skin, and muscle diseases. The scanner is the only instrument which Light Imaging Section staff is running for the users who get direct access to their images stored on a shared server. All slides have been scanned by Ms. Kenea.

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Project End
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Budget End
Support Year
10
Fiscal Year
2019
Total Cost
Indirect Cost
Name
National Institute of Arthritis and Musculoskeletal and Skin Diseases
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Iglesias-Bartolome, Ramiro; Uchiyama, Akihiko; Molinolo, Alfredo A et al. (2018) Transcriptional signature primes human oral mucosa for rapid wound healing. Sci Transl Med 10:
Sarshad, Aishe A; Juan, Aster H; Muler, Ana Iris Correa et al. (2018) Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells. Mol Cell :
Duverger, Olivier; Carlson, Jenna C; Karacz, Chelsea M et al. (2018) Genetic variants in pachyonychia congenita-associated keratins increase susceptibility to tooth decay. PLoS Genet 14:e1007168
Feng, Li Rebekah; Fernández-Martínez, Juan Luis; Zaal, Kristien J M et al. (2018) mGluR5 mediates post-radiotherapy fatigue development in cancer patients. Transl Psychiatry 8:110
Lee, Jeansun; Dieckmann, Nele M G; Edgar, James R et al. (2018) Fas Ligand localizes to intraluminal vesicles within NK cell cytolytic granules and is enriched at the immune synapse. Immun Inflamm Dis 6:312-321
Wolf, M; Ao, M; Chavez, M B et al. (2018) Reduced Orthodontic Tooth Movement in Enpp1 Mutant Mice with Hypercementosis. J Dent Res 97:937-945
Gupta, Sarthak; Chan, Diana W; Zaal, Kristien J et al. (2018) A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils. J Immunol 200:869-879
Thumbigere Math, V; Rebouças, P; Giovani, P A et al. (2018) Periodontitis in Chédiak-Higashi Syndrome: An Altered Immunoinflammatory Response. JDR Clin Trans Res 3:35-46
Bhattacharya, Shreya; Kim, Jin-Chul; Ogawa, Youichi et al. (2018) DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis. J Invest Dermatol 138:1052-1061
Sikora, Keith A; Bennett, Joshua R; Vyncke, Laurens et al. (2018) Germline gain-of-function myeloid differentiation primary response gene-88 (MYD88) mutation in a child with severe arthritis. J Allergy Clin Immunol 141:1943-1947.e9

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