Abstract

The HiTIF is currently involved in 22 collaborative projects with NCI intramural Research Investigators. The current HCI projects run at HiTIF cover many different basic research areas that are important for cancer research: - DNA Damage Response (DDR) - Chromosome Instability (CIN) - Cellular pathways involved in pediatric neuroblastoma - Epigenetics of DNA translocations - Nuclear hormone biology and regulation of gene expression - RNA splicing - Premature aging disease. The HiTIF is open to assist NCI and NIH investigators in the design, optimization, implementation and analysis of High-Content Imaging assays for the systematic study of cancer-relevant cellular phenotypes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC011567-05
Application #
9780256
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Zane, Linda; Chapus, Fleur; Pegoraro, Gianluca et al. (2017) HiHiMap: single-cell quantitation of histones and histone posttranslational modifications across the cell cycle by high-throughput imaging. Mol Biol Cell 28:2290-2302
Wang, Qiuyan; Sawyer, Iain A; Sung, Myong-Hee et al. (2016) Cajal bodies are linked to genome conformation. Nat Commun 7:10966
Pegoraro, Gianluca; Misteli, Tom (2016) High-throughput Imaging as a versatile and unbiased discovery tool. Methods 96:1-2
Stavreva, Diana A; Varticovski, Lyuba; Levkova, Ludmila et al. (2016) Novel cell-based assay for detection of thyroid receptor beta-interacting environmental contaminants. Toxicology 368-369:69-79
Ji, Xiong; Dadon, Daniel B; Powell, Benjamin E et al. (2016) 3D Chromosome Regulatory Landscape of Human Pluripotent Cells. Cell Stem Cell 18:262-75
Kubben, Nard; Zhang, Weiqi; Wang, Lixia et al. (2016) Repression of the Antioxidant NRF2 Pathway in Premature Aging. Cell 165:1361-1374
Burman, Bharat; Misteli, Tom; Pegoraro, Gianluca (2015) Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging. Genome Biol 16:146
Zong, Dali; Callén, Elsa; Pegoraro, Gianluca et al. (2015) Ectopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining. Nucleic Acids Res 43:4950-61
Roukos, Vassilis; Pegoraro, Gianluca; Voss, Ty C et al. (2015) Cell cycle staging of individual cells by fluorescence microscopy. Nat Protoc 10:334-48
Burman, Bharat; Zhang, Zhuzhu Z; Pegoraro, Gianluca et al. (2015) Histone modifications predispose genome regions to breakage and translocation. Genes Dev 29:1393-402

Showing the most recent 10 out of 12 publications