The work presented in this grant is focused on the long term goal of better understanding the roles of HSF1 and HSF2 in spermatogenesis and early embryogenesis. According to the Center for Disease Control (CDC) infertility affects approximately 6.1 million women and their partners in the United States. Defective expression of heat shock factors leads to several phenotypes including infertility in both males and females. To identify the role(s) of HSF1 and HSF2 in reproduction, these factors must be thoroughly characterized in vivo at various stages of spermatogenesis. The role of HSF2 bookmarking the hsp70i gene in mature spermatozoa must be evaluated. Finally, all gene targets bound by HSF1 and HSF2 must be identified. ? ?
|Wilkerson, Donald C; Sarge, Kevin D (2009) RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa. Reproduction 137:923-9|
|Murphy, Lynea A; Wilkerson, Donald C; Hong, Yiling et al. (2008) PRC1 associates with the hsp70i promoter and interacts with HSF2 during mitosis. Exp Cell Res 314:2224-30|
|Wilkerson, Donald C; Murphy, Lynea A; Sarge, Kevin D (2008) Interaction of HSF1 and HSF2 with the Hspa1b promoter in mouse epididymal spermatozoa. Biol Reprod 79:283-8|
|Wilkerson, Donald C; Skaggs, Hollie S; Sarge, Kevin D (2007) HSF2 binds to the Hsp90, Hsp27, and c-Fos promoters constitutively and modulates their expression. Cell Stress Chaperones 12:283-90|