The goal ofthis proposal is to establish a Stem Cell Core Facility (Core C) that will produce mouse bone marrow-derived mesenchymal stem cells (BMSCs) and muscle satellite cells and provide these cells to the individual projects involved in the Program Project. A key component essential to the success ofthe proposed Program Project is the availability of BMSCs because all projects require these cells. Thus, a centralized Core facility for the production of quality cells is crucial to the success of this dynamically coordinated Program Project. This Core will benefit the research efforts of individual investigator by reducing costs, providing quality control for study procedures, and enhancing collaborations among multiple disciplines to achieve greater scientific gain through an integrated approach. In addition, production and distribution of cells from a centralized facility will ensure that all studies start with cells ofthe same lineages and background. To achieve our stated goal, this proposal will pursue the following aims: 1. To isolate, characterize and distribute BMSCs and muscle satellite cells from C57BL/6 mice as well as from genetically modified mice (ob/ob, growth hormone deficient, and IGF-1 deficient mice) to individual projects for their use. 2. To provide technical support for cell culture, differentiation assays and analysis of gene expression. By achieving these objectives, this Core will function as a unique resource to provide mouse mesenchymal stem cells and technical assistance to the individual projects, as well as to other Medical College of Georgia investigators who are interested in studies related to aging, metabolic bone diseases, or stem cell research.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG036675-03
Application #
8463095
Study Section
Special Emphasis Panel (ZAG1-ZIJ-8)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
3
Fiscal Year
2013
Total Cost
$132,588
Indirect Cost
$44,196
Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912
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Herberg, Samuel; Kondrikova, Galina; Periyasamy-Thandavan, Sudharsan et al. (2014) Inkjet-based biopatterning of SDF-1? augments BMP-2-induced repair of critical size calvarial bone defects in mice. Bone 67:95-103
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Herberg, Samuel; Fulzele, Sadanand; Yang, Nianlan et al. (2013) Stromal cell-derived factor-1* potentiates bone morphogenetic protein-2-stimulated osteoinduction of genetically engineered bone marrow-derived mesenchymal stem cells in vitro. Tissue Eng Part A 19:1-13
Bowser, Matthew; Herberg, Samuel; Arounleut, Phonepasong et al. (2013) Effects of the activin A-myostatin-follistatin system on aging bone and muscle progenitor cells. Exp Gerontol 48:290-7

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