The goal ofthis proposal is to establish a Stem Cell Core Facility (Core C) that will produce mouse bone marrow-derived mesenchymal stem cells (BMSCs) and muscle satellite cells and provide these cells to the individual projects involved in the Program Project. A key component essential to the success ofthe proposed Program Project is the availability of BMSCs because all projects require these cells. Thus, a centralized Core facility for the production of quality cells is crucial to the success of this dynamically coordinated Program Project. This Core will benefit the research efforts of individual investigator by reducing costs, providing quality control for study procedures, and enhancing collaborations among multiple disciplines to achieve greater scientific gain through an integrated approach. In addition, production and distribution of cells from a centralized facility will ensure that all studies start with cells ofthe same lineages and background. To achieve our stated goal, this proposal will pursue the following aims: 1. To isolate, characterize and distribute BMSCs and muscle satellite cells from C57BL/6 mice as well as from genetically modified mice (ob/ob, growth hormone deficient, and IGF-1 deficient mice) to individual projects for their use. 2. To provide technical support for cell culture, differentiation assays and analysis of gene expression. By achieving these objectives, this Core will function as a unique resource to provide mouse mesenchymal stem cells and technical assistance to the individual projects, as well as to other Medical College of Georgia investigators who are interested in studies related to aging, metabolic bone diseases, or stem cell research.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG036675-03
Application #
8463095
Study Section
Special Emphasis Panel (ZAG1-ZIJ-8)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
3
Fiscal Year
2013
Total Cost
$132,588
Indirect Cost
$44,196
Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912
Kolhe, Ravindra; Mondal, Ashis K; Pundkar, Chetan et al. (2018) Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells. Nutrients 10:
Yu, Kanglun; Sellman, David P; Bahraini, Anoosh et al. (2018) Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone. J Orthop Res 36:653-662
Kesterke, Matthew J; Judd, Margaret A; Mooney, Mark P et al. (2018) Maternal environment and craniofacial growth: geometric morphometric analysis of mandibular shape changes with in utero thyroxine overexposure in mice. J Anat 233:46-54
Howie, R Nicole; Herberg, Samuel; Durham, Emily et al. (2018) Selective serotonin re-uptake inhibitor sertraline inhibits bone healing in a calvarial defect model. Int J Oral Sci 10:25
Elsayed, Ranya; Abraham, Pheba; Awad, Mohamed E et al. (2018) Removal of matrix-bound zoledronate prevents post-extraction osteonecrosis of the jaw by rescuing osteoclast function. Bone 110:141-149
Murphy, Cameron; Withrow, Joseph; Hunter, Monte et al. (2018) Emerging role of extracellular vesicles in musculoskeletal diseases. Mol Aspects Med 60:123-128
Roser-Page, Susanne; Vikulina, Tatyana; Yu, Kanglun et al. (2018) Neutralization of CD40 ligand costimulation promotes bone formation and accretion of vertebral bone mass in mice. Rheumatology (Oxford) 57:1105-1114
Bollag, Wendy B; Choudhary, Vivek; Zhong, Qing et al. (2018) Deletion of protein kinase D1 in osteoprogenitor cells results in decreased osteogenesis in vitro and reduced bone mineral density in vivo. Mol Cell Endocrinol 461:22-31
Kim, Beom-Jun; Lee, Seung Hun; Kwak, Mi Kyung et al. (2018) Inverse relationship between serum hsCRP concentration and hand grip strength in older adults: a nationwide population-based study. Aging (Albany NY) 10:2051-2061
Kim, B-J; Lee, S H; Isales, C M et al. (2018) The positive association of total protein intake with femoral neck strength (KNHANES IV). Osteoporos Int 29:1397-1405

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