The goal ofthis proposal is to establish a Stem Cell Core Facility (Core C) that will produce mouse bone marrow-derived mesenchymal stem cells (BMSCs) and muscle satellite cells and provide these cells to the individual projects involved in the Program Project. A key component essential to the success ofthe proposed Program Project is the availability of BMSCs because all projects require these cells. Thus, a centralized Core facility for the production of quality cells is crucial to the success of this dynamically coordinated Program Project. This Core will benefit the research efforts of individual investigator by reducing costs, providing quality control for study procedures, and enhancing collaborations among multiple disciplines to achieve greater scientific gain through an integrated approach. In addition, production and distribution of cells from a centralized facility will ensure that all studies start with cells ofthe same lineages and background. To achieve our stated goal, this proposal will pursue the following aims: 1. To isolate, characterize and distribute BMSCs and muscle satellite cells from C57BL/6 mice as well as from genetically modified mice (ob/ob, growth hormone deficient, and IGF-1 deficient mice) to individual projects for their use. 2. To provide technical support for cell culture, differentiation assays and analysis of gene expression. By achieving these objectives, this Core will function as a unique resource to provide mouse mesenchymal stem cells and technical assistance to the individual projects, as well as to other Medical College of Georgia investigators who are interested in studies related to aging, metabolic bone diseases, or stem cell research.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG036675-03
Application #
8463095
Study Section
Special Emphasis Panel (ZAG1-ZIJ-8)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
3
Fiscal Year
2013
Total Cost
$132,588
Indirect Cost
$44,196
Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912
López, Melany; Bollag, Roni J; Yu, Jack C et al. (2016) Chemically Defined and Xeno-Free Cryopreservation of Human Adipose-Derived Stem Cells. PLoS One 11:e0152161
Howie, R Nicole; Durham, Emily L; Black, Laurel et al. (2016) Effects of In Utero Thyroxine Exposure on Murine Cranial Suture Growth. PLoS One 11:e0167805
Withrow, Joseph; Murphy, Cameron; Liu, Yutao et al. (2016) Extracellular vesicles in the pathogenesis of rheumatoid arthritis and osteoarthritis. Arthritis Res Ther 18:286
Durham, Emily L; Howie, R Nicole; Black, Laurel et al. (2016) Effects of thyroxine exposure on the Twist 1 +/- phenotype: A test of gene-environment interaction modeling for craniosynostosis. Birth Defects Res A Clin Mol Teratol 106:803-813
Bhatta, Anil; Sangani, Rajnikumar; Kolhe, Ravindra et al. (2016) Deregulation of arginase induces bone complications in high-fat/high-sucrose diet diabetic mouse model. Mol Cell Endocrinol 422:211-20
Hamrick, Mark W; McGee-Lawrence, Meghan E; Frechette, Danielle M (2016) Fatty Infiltration of Skeletal Muscle: Mechanisms and Comparisons with Bone Marrow Adiposity. Front Endocrinol (Lausanne) 7:69
Sangani, Rajnikumar; Periyasamy-Thandavan, Sudharsan; Pathania, Rajneesh et al. (2015) The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis. Stem Cell Res 15:312-21
El Refaey, Mona; Watkins, Christopher P; Kennedy, Eileen J et al. (2015) Oxidation of the aromatic amino acids tryptophan and tyrosine disrupts their anabolic effects on bone marrow mesenchymal stem cells. Mol Cell Endocrinol 410:87-96
Herberg, Samuel; Aguilar-Perez, Alexandra; Howie, R Nicole et al. (2015) Mesenchymal stem cell expression of SDF-1β synergizes with BMP-2 to augment cell-mediated healing of critical-sized mouse calvarial defects. J Tissue Eng Regen Med :
Herberg, Samuel; Kondrikova, Galina; Hussein, Khaled A et al. (2015) Mesenchymal stem cell expression of stromal cell-derived factor-1β augments bone formation in a model of local regenerative therapy. J Orthop Res 33:174-84

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