leader's approved protocol to: 1) anesthetize the mice, 2) perform saline perfusion, which euthanizes the mouse by exsanguination, 3) dissect the mouse to display the mouse aorta in situ, 4) remove the aorta and trim off adventitial tissue, 5) cut the aorta longitudinally and fix it flattened between two microscope slides, 6) stain the aorta with oil red O, 7) mount the aorta on a microscope slide, and 8) quantify the % surface lesion area in the entire aorta, as well as in the regions of the aortic arch, and thoracic and abdominal aortas. Utilization of the Core Facility

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL029582-29
Application #
8378192
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
29
Fiscal Year
2012
Total Cost
$261,118
Indirect Cost
$94,222
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
Liu, Caini; Zhu, Liang; Fukuda, Koichi et al. (2017) The flavonoid cyanidin blocks binding of the cytokine interleukin-17A to the IL-17RA subunit to alleviate inflammation in vivo. Sci Signal 10:
Aziz, Moammir H; Cui, Kui; Das, Mitali et al. (2017) The Upregulation of Integrin ?D?2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis. J Immunol 198:4855-4867
Arif, Abul; Terenzi, Fulvia; Potdar, Alka A et al. (2017) EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice. Nature 542:357-361
Shao, Xinrui; Chen, Siyuan; Yang, Daping et al. (2017) FGF2 cooperates with IL-17 to promote autoimmune inflammation. Sci Rep 7:7024
Arif, Abul; Jia, Jie; Halawani, Dalia et al. (2017) Experimental approaches for investigation of aminoacyl tRNA synthetase phosphorylation. Methods 113:72-82
Israel, Laura; Wang, Ying; Bulek, Katarzyna et al. (2017) Human Adaptive Immunity Rescues an Inborn Error of Innate Immunity. Cell 168:789-800.e10
Zhou, Hao; Yu, Minja; Roychowdhury, Sanjoy et al. (2017) Myeloid-MyD88 Contributes to Ethanol-Induced Liver Injury in Mice Linking Hepatocellular Death to Inflammation. Alcohol Clin Exp Res 41:719-726
Zhou, Hao; Bulek, Katarzyna; Li, Xiao et al. (2017) IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs. Elife 6:
Arif, Abul; Yao, Peng; Terenzi, Fulvia et al. (2017) The GAIT translational control system. Wiley Interdiscip Rev RNA :
Wang, Chenhui; Zhang, Cun-Jin; Martin, Bradley N et al. (2017) IL-17 induced NOTCH1 activation in oligodendrocyte progenitor cells enhances proliferation and inflammatory gene expression. Nat Commun 8:15508

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