Abstract

The purpose of this core is to provide service to the subprojects in the preparation of protein reagents. Most of the work will be conducted in the Muscle Biology Laboratory which has approximately 2000 square feet, including a walk-in cold room, that can be devoted to core activities. In addition this laboratory has a full abattoir at the other end of the building where tissue from large animals (bovine, pig, sheep) can be obtained immediately post-mortem. Protein purification is time consuming and requires a variety of expensive equipment (centrifuges, spectrophotometers, chromatography columns, HPLC, fraction collectors, etc.) that are hard to justify if protein purification needs are sporadic. In addition a number of proteins will be used by several projects concurrently, so sharing of these reagents leads to overall improvement in experimental efficiency. The core director has many years of hands-on experience in protein purification and antibody production and thus can capably direct the core effort.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL047053-03
Application #
6242161
Study Section
Project Start
1997-01-01
Project End
1997-12-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Druckenbrod, Noah R; Powers, Patricia A; Bartley, Christopher R et al. (2008) Targeting of endothelin receptor-B to the neural crest. Genesis 46:396-400
Brickson, S; Fitzsimons, D P; Pereira, L et al. (2007) In vivo left ventricular functional capacity is compromised in cMyBP-C null mice. Am J Physiol Heart Circ Physiol 292:H1747-54
Vatta, Matteo; Ackerman, Michael J; Ye, Bin et al. (2006) Mutant caveolin-3 induces persistent late sodium current and is associated with long-QT syndrome. Circulation 114:2104-12
Stelzer, Julian E; Larsson, Lars; Fitzsimons, Daniel P et al. (2006) Activation dependence of stretch activation in mouse skinned myocardium: implications for ventricular function. J Gen Physiol 127:95-107
Stelzer, Julian E; Fitzsimons, Daniel P; Moss, Richard L (2006) Ablation of myosin-binding protein-C accelerates force development in mouse myocardium. Biophys J 90:4119-27
Singla, Dinender K; Hacker, Timothy A; Ma, Lining et al. (2006) Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. J Mol Cell Cardiol 40:195-200
Muthukumarana, Poorni A D S; Lyons, Gary E; Miura, Yuji et al. (2006) Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance. Int Immunopharmacol 6:1993-2001
Stelzer, Julian E; Patel, Jitandrakumar R; Moss, Richard L (2006) Acceleration of stretch activation in murine myocardium due to phosphorylation of myosin regulatory light chain. J Gen Physiol 128:261-72
Stelzer, Julian E; Patel, Jitandrakumar R; Moss, Richard L (2006) Protein kinase A-mediated acceleration of the stretch activation response in murine skinned myocardium is eliminated by ablation of cMyBP-C. Circ Res 99:884-90
Balijepalli, Ravi C; Foell, Jason D; Hall, Duane D et al. (2006) Localization of cardiac L-type Ca(2+) channels to a caveolar macromolecular signaling complex is required for beta(2)-adrenergic regulation. Proc Natl Acad Sci U S A 103:7500-5

Showing the most recent 10 out of 103 publications