Studies proposed in Project 3 will investigate the fundamental question that Ca2+ entry mediated by transient receptor potential cation channel 6 (TRPC6), a receptor-operated channel (ROC), is a critical determinant of increased endothelial permeability. We will test the hypothesis that TRPC6 is the critical influx pathway for Ca2+ that triggers the activation of endothelial contractile mechanisms RhoA and endothelial isoform of MLCK (EC-MLCK). If this hypothesis is correct, it would demonstrate a fundamental link between TRPC6 and the RhoA-EC-MLCK pathway. We also postulate based on our Supporting Data a potentially important relationship by which TRPC6-activated signaling upregulates the activity of TRPC1, a store-operated channel (SOC). Thus, our concept is that TRPC6 by activating both RhoA and EC-MLCK controls the state of endothelial contraction and additionally that TRPC6 activates signals that upregulate TRPC1 activity, thereby protracting and/or amplifying the endothelial permeability response.
Our Specific Aims are to determine: 1) the role of the endothelial ROC, TRPC6, interaction with the SOC, TRPC1, in mediating Ca2+ signaling-dependent increase in lung vascular permeability;2) the role of TRPC6-activated signaling in mediafing RhoA and ECMLCK activation and the requirement of these effectors in mediating increased lung vascular endothelial permeability and 3) the role of EC-MLCK downstream of TRPC6 in upregulating TRPC1 activity, and its consequences in increasing lung endothelial permeability. These studies will be carried out utilizing endothelial cells and lungs from mice carrying deletions of specific genes. We believe that the studies will provide a new understanding of the Ca2+-mediated increase in lung vascular permeability and edema formation. These new findings will help to unravel the details of how the interactions between TRPC6 and TRPC1 signals the Ca2+-dependent increase in lung endothelial permeability. The studies are expected to offer a novel therapeutic strategy for inhibiting TRPC6 and TRPC1 activities, thereby abrogating or reversing lung vascular leakiness.

Public Health Relevance

A rise in intracellular calcium by activating cell contraction leads to formation of pores between endothelial cells through which blood proteins can gain access to interstitium and impair gas exchange in lungs thereby leading to acute lung injury (ALI). We believe proposed studies will provide novel insights into the role of a calcium cahnnel TRPC6 in inducing ALI and will identify TRPC6 as a therapeutic target to prevent ALI.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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University of Illinois at Chicago
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Di, Anke; Mehta, Dolly; Malik, Asrar B (2016) ROS-activated calcium signaling mechanisms regulating endothelial barrier function. Cell Calcium 60:163-71
Tauseef, Mohammad; Farazuddin, Mohammad; Sukriti, Sukriti et al. (2016) Transient receptor potential channel 1 maintains adherens junction plasticity by suppressing sphingosine kinase 1 expression to induce endothelial hyperpermeability. FASEB J 30:102-10
Gong, Haixia; An, Shejuan; Sassmann, Antonia et al. (2016) PAR1 Scaffolds TGFβRII to Downregulate TGF-β Signaling and Activate ESC Differentiation to Endothelial Cells. Stem Cell Reports 7:1050-1058
Rajput, Charu; Tauseef, Mohammad; Farazuddin, Mohammad et al. (2016) MicroRNA-150 Suppression of Angiopoetin-2 Generation and Signaling Is Crucial for Resolving Vascular Injury. Arterioscler Thromb Vasc Biol 36:380-8
Ebenezer, David L; Fu, Panfeng; Suryadevara, Vidyani et al. (2016) Epigenetic regulation of pro-inflammatory cytokine secretion by sphingosine 1-phosphate (S1P) in acute lung injury: Role of S1P lyase. Adv Biol Regul :
Zimnicka, Adriana M; Husain, Yawer S; Shajahan, Ayesha N et al. (2016) Src-dependent phosphorylation of caveolin-1 Tyr-14 promotes swelling and release of caveolae. Mol Biol Cell 27:2090-106
Jiang, Ying; Sverdlov, Maria S; Toth, Peter T et al. (2016) Phosphatidic Acid Produced by RalA-activated PLD2 Stimulates Caveolae-mediated Endocytosis and Trafficking in Endothelial Cells. J Biol Chem 291:20729-38
Liu, Yuru; Kumar, Varsha Suresh; Zhang, Wei et al. (2015) Activation of type II cells into regenerative stem cell antigen-1(+) cells during alveolar repair. Am J Respir Cell Mol Biol 53:113-24
Geyer, Melissa; Huang, Fei; Sun, Ying et al. (2015) Microtubule-Associated Protein EB3 Regulates IP3 Receptor Clustering and Ca(2+) Signaling in Endothelial Cells. Cell Rep 12:79-89
Zhang, Xianming; Brovkovych, Viktor; Zhang, Yongkang et al. (2015) Downregulation of kinin B1 receptor function by B2 receptor heterodimerization and signaling. Cell Signal 27:90-103

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