Abstract

The genes for histidine utilization (hut) in Klebsiella aerogenes are typical of a large number of genes whose expression is strictly regulated by the available supply of nitrogen. Thus nitrogen regulation of hut serves as a model system for this kind of pleiotropic control. The goal of the research proposed here is to develop an in vitro transcription system for the hut operon of K. aerogenes that is responsive to regulation by the nitrogen regulatory factor(s) and to study the molecular basis for that regulation. The specific objectives of the five year proposal period are: (1) Physical characterization of the regulatory region (hutP) including the DNA sequence of the region and identification of the sites to which regulatory proteins bind; (2) Genetic analysis of the hutP region, including isolation and mapping of hut mutants unable to respond to nitrogen regulation; (3) Characterization of genetic factors required for nitrogen regulation of hutP expression; and (4) Regulation of transcription from hutP in vitro, including regulation by CAP+cAMP and purification of the nitrogen activating protein.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI015822-06
Application #
3126441
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1979-05-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
Schools of Arts and Sciences
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Osuna, R; Janes, B K; Bender, R A (1994) Roles of catabolite activator protein sites centered at -81.5 and -41.5 in the activation of the Klebsiella aerogenes histidine utilization operon hutUH. J Bacteriol 176:5513-24
Osuna, R; Bender, R A (1991) Klebsiella aerogenes catabolite gene activator protein and the gene encoding it (crp). J Bacteriol 173:6626-31
Osuna, R; Boylan, S A; Bender, R A (1991) In vitro transcription of the histidine utilization (hutUH) operon from Klebsiella aerogenes. J Bacteriol 173:116-23
Best, E A; Bender, R A (1990) Cloning of the Klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in Salmonella typhimurium. J Bacteriol 172:7043-8
Bender, R A; Friedrich, B (1990) Regulation of assimilatory nitrate reductase formation in Klebsiella aerogenes W70. J Bacteriol 172:7256-9
Macaluso, A; Best, E A; Bender, R A (1990) Role of the nac gene product in the nitrogen regulation of some NTR-regulated operons of Klebsiella aerogenes. J Bacteriol 172:7249-55
Schwacha, A; Bender, R A (1990) Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Klebsiella aerogenes. J Bacteriol 172:5477-81
Schwacha, A; Cohen, J A; Gehring, K B et al. (1990) Tn1000-mediated insertion mutagenesis of the histidine utilization (hut) gene cluster from Klebsiella aerogenes: genetic analysis of hut and unusual target specificity of Tn1000. J Bacteriol 172:5991-8
Bender, R A; Refson, C M; O'Neill, E A (1989) Role of the flagellum in cell-cycle-dependent expression of bacteriophage receptor activity in Caulobacter crescentus. J Bacteriol 171:1035-40
O'Neill, E A; Bender, R A (1989) Cell-cycle-dependent polar morphogenesis in Caulobacter crescentus: roles of phospholipid, DNA, and protein syntheses. J Bacteriol 171:4814-20

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