The Bioassay Core will provide biochemical assay, FAGS analyses, histological, and immunohistochemical services for the individual projects within the Program Project. Bioassays that will be performed include analyses in blood, plasma, tissue, and urine for human, rat, and mouse samples. The following assays will be performed: Plasma renin activity, angiotensin II, aldosterone, nitrite/nitrate, cGMP, 8-isoprostane, antioxidant capacity, TSARS, albumin, creatinine, electrolytes, lipid panels, metabolic panels, endothelin-1 (ET-1), leptin, adiponectin, insulin, glucose, norepinephrine, epinephrine, and inflammatory markers including IL1. MCP-1, siCAM, CRP, IL6, IL10, TNFa, siCAM, TNFR1, TNFR2. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, colorimetric and fluorescent ELISA, FAGS analyses of lymphocytes and mononuclear cells, and ion-selective electrode analyses as well as ET receptor binding assays. Moreover, the Bioassay Core will perform histological analyses including H&E staining and immunohistochemistry of renal tissue sections. The core will also provide resources for development of additional assays as needed by individual investigators. Quality control of all assays, improvement of techniques, and establishment of reliability and validity of new techniques, maintaining records and data entry of all results to the projects will be handled by the Bioassay Core. This will lead to increased productivity among the projects of the Program Project.
to Public Health: Essential hypertension is the number 1 reason for a physician visit, with 1 in 3 people suffering from the disorder. This knowledge will enable us to develop more effective prevention and treatment strategies. Measurements of important biomolecules in tissues and fluids derived from human and animal studies in this program project provides important diagnostic information that allows us to understand the pathogenesis of disease.
|Pollock, David M (2014) 2013 Dahl Lecture: American Heart Association council for high blood pressure research clarifying the physiology of endothelin. Hypertension 63:e110-7|
|Su, Shaoyong; Zhu, Haidong; Xu, Xiaojing et al. (2014) DNA methylation of the LY86 gene is associated with obesity, insulin resistance, and inflammation. Twin Res Hum Genet 17:183-91|
|Riese, Harriëtte; Muñoz, Loretto M; Hartman, Catharina A et al. (2014) Identifying genetic variants for heart rate variability in the acetylcholine pathway. PLoS One 9:e112476|
|Hyndman, Kelly A; Ho, Dao H; Sega, Martiana F et al. (2014) Histone deacetylase 1 reduces NO production in endothelial cells via lysine deacetylation of NO synthase 3. Am J Physiol Heart Circ Physiol 307:H803-9|
|Su, Shaoyong; Wang, Xiaoling; Kapuku, Gaston K et al. (2014) Adverse childhood experiences are associated with detrimental hemodynamics and elevated circulating endothelin-1 in adolescents and young adults. Hypertension 64:201-7|
|Jin, Chunhua; Jeon, Yejoo; Kleven, Daniel T et al. (2014) Combined endothelin a blockade and chlorthalidone treatment in a rat model of metabolic syndrome. J Pharmacol Exp Ther 351:467-73|
|Kittikulsuth, W; Sullivan, J C; Pollock, D M (2013) ET-1 actions in the kidney: evidence for sex differences. Br J Pharmacol 168:318-26|
|Loria, Analia S; Brands, Michael W; Pollock, David M et al. (2013) Early life stress sensitizes the renal and systemic sympathetic system in rats. Am J Physiol Renal Physiol 305:F390-5|
|Spradley, F T; Kang, K-T; Pollock, J S (2013) Short-term hypercaloric diet induces blunted aortic vasoconstriction and enhanced vasorelaxation via increased nitric oxide synthase 3 activity and expression in Dahl salt-sensitive rats. Acta Physiol (Oxf) 207:358-68|
|Kittikulsuth, Wararat; Looney, Stephen W; Pollock, David M (2013) Endothelin ET(B) receptors contribute to sex differences in blood pressure elevation in angiotensin II hypertensive rats on a high-salt diet. Clin Exp Pharmacol Physiol 40:362-70|
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