The Bioassay Core will provide biochemical assay, FAGS analyses, histological, and immunohistochemical services for the individual projects within the Program Project. Bioassays that will be performed include analyses in blood, plasma, tissue, and urine for human, rat, and mouse samples. The following assays will be performed: Plasma renin activity, angiotensin II, aldosterone, nitrite/nitrate, cGMP, 8-isoprostane, antioxidant capacity, TSARS, albumin, creatinine, electrolytes, lipid panels, metabolic panels, endothelin-1 (ET-1), leptin, adiponectin, insulin, glucose, norepinephrine, epinephrine, and inflammatory markers including IL1. MCP-1, siCAM, CRP, IL6, IL10, TNFa, siCAM, TNFR1, TNFR2. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, colorimetric and fluorescent ELISA, FAGS analyses of lymphocytes and mononuclear cells, and ion-selective electrode analyses as well as ET receptor binding assays. Moreover, the Bioassay Core will perform histological analyses including H&E staining and immunohistochemistry of renal tissue sections. The core will also provide resources for development of additional assays as needed by individual investigators. Quality control of all assays, improvement of techniques, and establishment of reliability and validity of new techniques, maintaining records and data entry of all results to the projects will be handled by the Bioassay Core. This will lead to increased productivity among the projects of the Program Project.
to Public Health: Essential hypertension is the number 1 reason for a physician visit, with 1 in 3 people suffering from the disorder. This knowledge will enable us to develop more effective prevention and treatment strategies. Measurements of important biomolecules in tissues and fluids derived from human and animal studies in this program project provides important diagnostic information that allows us to understand the pathogenesis of disease.
|Kang, Kyu-Tae; Sullivan, Jennifer C; Pollock, Jennifer S (2018) Superoxide Dismutase Activity in Small Mesenteric Arteries Is Downregulated by Angiotensin II but Not by Hypertension. Toxicol Res 34:363-370|
|Johnston, Jermaine G; Pollock, David M (2018) Circadian regulation of renal function. Free Radic Biol Med 119:93-107|
|Hao, G; Wang, X; Treiber, F A et al. (2018) Body mass index trajectories in childhood is predictive of cardiovascular risk: results from the 23-year longitudinal Georgia Stress and Heart study. Int J Obes (Lond) 42:923-925|
|Mathur, Shreya; Pollock, Jennifer S; Mathur, Sunil et al. (2018) Relation of urinary endothelin-1 to stress-induced pressure natriuresis in healthy adolescents. J Am Soc Hypertens 12:34-41|
|Kapuku, G; Treiber, F; Raouane, F et al. (2017) Race/ethnicity determines the relationships between oxidative stress markers and blood pressure in individuals with high cardiovascular disease risk. J Hum Hypertens 31:70-75|
|Hao, Guang; Wang, Xiaoling; Treiber, Frank A et al. (2017) Blood Pressure Trajectories From Childhood to Young Adulthood Associated With Cardiovascular Risk: Results From the 23-Year Longitudinal Georgia Stress and Heart Study. Hypertension 69:435-442|
|Stewart, Deborah; Dong, Yanbin; Zhu, Haidong et al. (2017) Angiotensin II-Mediated Increases in Damage-Associated Molecular Patterns During Acute Mental Stress. Psychosom Med 79:112-114|
|Youssef, Nagy A; Belew, Daniel; Hao, Guang et al. (2017) Racial/ethnic differences in the association of childhood adversities with depression and the role of resilience. J Affect Disord 208:577-581|
|De Miguel, Carmen; Speed, Joshua S; Kasztan, Malgorzata et al. (2016) Endothelin-1 and the kidney: new perspectives and recent findings. Curr Opin Nephrol Hypertens 25:35-41|
|Gohar, Eman Y; Giachini, Fernanda R; Pollock, David M et al. (2016) Role of the endothelin system in sexual dimorphism in cardiovascular and renal diseases. Life Sci 159:20-29|
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