The Bioassay Core will provide biochemical assay, FAGS analyses, histological, and immunohistochemical services for the individual projects within the Program Project. Bioassays that will be performed include analyses in blood, plasma, tissue, and urine for human, rat, and mouse samples. The following assays will be performed: Plasma renin activity, angiotensin II, aldosterone, nitrite/nitrate, cGMP, 8-isoprostane, antioxidant capacity, TSARS, albumin, creatinine, electrolytes, lipid panels, metabolic panels, endothelin-1 (ET-1), leptin, adiponectin, insulin, glucose, norepinephrine, epinephrine, and inflammatory markers including IL1. MCP-1, siCAM, CRP, IL6, IL10, TNFa, siCAM, TNFR1, TNFR2. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, colorimetric and fluorescent ELISA, FAGS analyses of lymphocytes and mononuclear cells, and ion-selective electrode analyses as well as ET receptor binding assays. Moreover, the Bioassay Core will perform histological analyses including H&E staining and immunohistochemistry of renal tissue sections. The core will also provide resources for development of additional assays as needed by individual investigators. Quality control of all assays, improvement of techniques, and establishment of reliability and validity of new techniques, maintaining records and data entry of all results to the projects will be handled by the Bioassay Core. This will lead to increased productivity among the projects of the Program Project.

Public Health Relevance

to Public Health: Essential hypertension is the number 1 reason for a physician visit, with 1 in 3 people suffering from the disorder. This knowledge will enable us to develop more effective prevention and treatment strategies. Measurements of important biomolecules in tissues and fluids derived from human and animal studies in this program project provides important diagnostic information that allows us to understand the pathogenesis of disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL069999-11A1
Application #
8701538
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
11
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Georgia Regents University
Department
Type
DUNS #
City
Augusta
State
GA
Country
United States
Zip Code
30912
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