The Bioassay Core will provide biochemical assay, FAGS analyses, histological, and immunohistochemical services for the individual projects within the Program Project. Bioassays that will be performed include analyses in blood, plasma, tissue, and urine for human, rat, and mouse samples. The following assays will be performed: Plasma renin activity, angiotensin II, aldosterone, nitrite/nitrate, cGMP, 8-isoprostane, antioxidant capacity, TSARS, albumin, creatinine, electrolytes, lipid panels, metabolic panels, endothelin-1 (ET-1), leptin, adiponectin, insulin, glucose, norepinephrine, epinephrine, and inflammatory markers including IL1. MCP-1, siCAM, CRP, IL6, IL10, TNFa, siCAM, TNFR1, TNFR2. Techniques will include radioimmunoassay, radioenzymatic assay, HPLC, colorimetric and fluorescent ELISA, FAGS analyses of lymphocytes and mononuclear cells, and ion-selective electrode analyses as well as ET receptor binding assays. Moreover, the Bioassay Core will perform histological analyses including H&E staining and immunohistochemistry of renal tissue sections. The core will also provide resources for development of additional assays as needed by individual investigators. Quality control of all assays, improvement of techniques, and establishment of reliability and validity of new techniques, maintaining records and data entry of all results to the projects will be handled by the Bioassay Core. This will lead to increased productivity among the projects of the Program Project.

Public Health Relevance

to Public Health: Essential hypertension is the number 1 reason for a physician visit, with 1 in 3 people suffering from the disorder. This knowledge will enable us to develop more effective prevention and treatment strategies. Measurements of important biomolecules in tissues and fluids derived from human and animal studies in this program project provides important diagnostic information that allows us to understand the pathogenesis of disease.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
2P01HL069999-11A1
Application #
8701538
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Georgia Regents University
Department
Type
DUNS #
City
Augusta
State
GA
Country
United States
Zip Code
30912
De Miguel, Carmen; Speed, Joshua S; Kasztan, Malgorzata et al. (2016) Endothelin-1 and the kidney: new perspectives and recent findings. Curr Opin Nephrol Hypertens 25:35-41
Heimlich, J Brett; Speed, Joshua S; O'Connor, Paul M et al. (2016) Endothelin-1 contributes to the progression of renal injury in sickle cell disease via reactive oxygen species. Br J Pharmacol 173:386-95
Davenport, Anthony P; Hyndman, Kelly A; Dhaun, Neeraj et al. (2016) Endothelin. Pharmacol Rev 68:357-418
Gohar, Eman Y; Giachini, Fernanda R; Pollock, David M et al. (2016) Role of the endothelin system in sexual dimorphism in cardiovascular and renal diseases. Life Sci 159:20-9
Spradley, Frank T; Ho, Dao H; Pollock, Jennifer S (2016) Dahl SS rats demonstrate enhanced aortic perivascular adipose tissue-mediated buffering of vasoconstriction through activation of NOS in the endothelium. Am J Physiol Regul Integr Comp Physiol 310:R286-96
Guan, Zhengrong; Singletary, Sean T; Cha, Haword et al. (2016) Pentosan polysulfate preserves renal microvascular P2X1 receptor reactivity and autoregulatory behavior in DOCA-salt hypertensive rats. Am J Physiol Renal Physiol 310:F456-65
Heimlich, J B; Speed, J S; Bloom, C J et al. (2015) ET-1 increases reactive oxygen species following hypoxia and high-salt diet in the mouse glomerulus. Acta Physiol (Oxf) 213:722-30
Su, Shaoyong; Wang, Xiaoling; Pollock, Jennifer S et al. (2015) Adverse childhood experiences and blood pressure trajectories from childhood to young adulthood: the Georgia stress and Heart study. Circulation 131:1674-81
Speed, Joshua S; Fox, Brandon M; Johnston, Jermaine G et al. (2015) Endothelin and renal ion and water transport. Semin Nephrol 35:137-44
Speed, Joshua S; Pollock, David M (2015) New clues towards solving the mystery of endothelin and blood pressure regulation. Hypertension 66:275-7

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