The Cell Culture Core, designated as Core B, is a centralized facility that provides primary alveolar epithelial type 2 and type 1 cells from rats and mice to each of the four projects and to Core C. Additionally, the Core provides primary derived fibroblasts and maintains secondary alveolar epithelial cell lines for the PPG. Centralization of the cell culture facilities will ensure that a continuous supply of high quality alveolar epithelial cells is available to each of the four projects. The cell culture facility personnel has extensive experience in the isolation of primary alveolar type 2 and type 1 cells from rats and mice, as well as general cell culture techniques. The consolidation of the cell culture facilities will provide an economical means of isolating and culturing alveolar epithelial cells. This translates into reduced overall costs (i.e. personnel, reagents, animals) and more importantly maintains the precision and accuracy with which the cells are isolated and cultured

Public Health Relevance

Currently, there are no established cell lines that maintain the phenotypical characteristics of primary alveolar epithelial cells. Consequently, it is necessary to isolate the alveolar epithelial cells from the lungs of rodents so that these cells can be used by the Principal Investigator's of each of the four projects to directly test their hypotheses. Primary alveolar epithelial cells best reflect the in vivo environment of the alveolar epithelium

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL071643-09
Application #
8376678
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
9
Fiscal Year
2012
Total Cost
$354,541
Indirect Cost
$122,055
Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
Sala, Marc A; Balderas-Martínez, Yalbi Itzel; Buendía-Roldan, Ivette et al. (2018) Inflammatory pathways are upregulated in the nasal epithelium in patients with idiopathic pulmonary fibrosis. Respir Res 19:233
Mehta, Manan M; Weinberg, Samuel E; Steinert, Elizabeth M et al. (2018) Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab 6:10
Brazee, Patricia L; Dada, Laura A (2018) Splice Wars: The Role of MLCK Isoforms in Ventilation-induced Lung Injury. Am J Respir Cell Mol Biol 58:549-550
Koch, Clarissa M; Chiu, Stephen F; Akbarpour, Mahzad et al. (2018) A Beginner's Guide to Analysis of RNA Sequencing Data. Am J Respir Cell Mol Biol 59:145-157
Dela Cruz, Charles S; Wunderink, Richard G; Christiani, David C et al. (2018) Future Research Directions in Pneumonia. NHLBI Working Group Report. Am J Respir Crit Care Med 198:256-263
Kong, Hyewon; Chandel, Navdeep S (2018) Regulation of redox balance in cancer and T cells. J Biol Chem 293:7499-7507
Hsiao, Hsi-Min; Fernandez, Ramiro; Tanaka, Satona et al. (2018) Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1?. J Clin Invest 128:2833-2847
Wang, Zheng; Divanyan, Alex; Jourd'heuil, Frances L et al. (2018) Vimentin expression is required for the development of EMT-related renal fibrosis following unilateral ureteral obstruction in mice. Am J Physiol Renal Physiol 315:F769-F780
Lu, Ziyan; Casalino-Matsuda, S Marina; Nair, Aisha et al. (2018) A role for heat shock factor 1 in hypercapnia-induced inhibition of inflammatory cytokine expression. FASEB J 32:3614-3622
Amarelle, Luciano; Lecuona, Emilia (2018) A Nonhospitable Host: Targeting Cellular Factors as an Antiviral Strategy for Respiratory Viruses. Am J Respir Cell Mol Biol 59:666-667

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