The Non-human primate Core will provide five essential research support services for all research Projects. First, the Core will manage and provide oversight of all aspects of non-human primate screening, procurement, and compliance with federal animal welfare and biosafety regulations and University guidelines. Second, the Core will oversee husbandry and veterinary care for the non-human primates of each ofthe three Projects and provide clinical evaluations throughout the studies. Third, the Core will provide technical expertise in experimental infection of macaques with SHIV and Pneumocystis, procedures and manipulations such as immunizations, inoculations, blood sample collection, bronchoscopy, pulmonary function testing and imaging, in support ofthe individual research Projects. Fourth, the Core will provide flow cytometry and cellular immunology services of primate samples. Fifth, the Core will perform necropsies and provide tissues preparation and banking at termination, collect, manage and distribute serial samples from primates in each of the studies and will coordinate data analyses with Project Pis. Centralizing all nonhuman primate work will standardize animal procedures and assure consistency in animal care, sample collection and analyses among the groups of animals used in the individual projects.

Public Health Relevance

Infection with Pneumocystis remains a leading cause of morbidity and mortality in immunocompromised patients, including HIV-infected individuals. This work is directly relevant to the evaluation of Pneumocystis vaccine strategies in healthy and SHIV-immunosuppressed macaques. Pre-clinical testing of prophylactic and therapeutic vaccination strategies in a primate model of HIV infection is an essential step toward eventual clinical trials.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL076100-10
Application #
8708939
Study Section
Special Emphasis Panel (ZHL1-PPG-A)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
10
Fiscal Year
2014
Total Cost
$667,280
Indirect Cost
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Zheng, Mingquan; Cai, Yang; Eddens, Taylor et al. (2014) Novel pneumocystis antigen discovery using fungal surface proteomics. Infect Immun 82:2417-23
Chakraborty, Sourav; Cai, Yang; Tarr, Matthew A (2014) In vitro oxidative footprinting provides insight into apolipoprotein B-100 structure in low-density lipoprotein. Proteomics 14:2614-22
D'Souza, Alain J; Desai, Shyamal D; Rudner, Xiaowen L et al. (2013) Suppression of the macrophage proteasome by ethanol impairs MHC class I antigen processing and presentation. PLoS One 8:e56890
Kelly, Michelle N; Zheng, Mingquan; Ruan, Sanbao et al. (2013) Memory CD4+ T cells are required for optimal NK cell effector functions against the opportunistic fungal pathogen Pneumocystis murina. J Immunol 190:285-95
Patil, Ujwal S; Qu, Haiou; Caruntu, Daniela et al. (2013) Labeling primary amine groups in peptides and proteins with N-hydroxysuccinimidyl ester modified Fe3O4@SiO2 nanoparticles containing cleavable disulfide-bond linkers. Bioconjug Chem 24:1562-9
Porretta, Elizabeth; Happel, Kyle I; Teng, Xu S et al. (2012) The impact of alcohol on BCG-induced immunity against Mycobacterium tuberculosis. Alcohol Clin Exp Res 36:310-7
Auten, Matthew W; Huang, Weitao; Dai, Guixiang et al. (2012) CD40 ligand enhances immunogenicity of vector-based vaccines in immunocompetent and CD4+ T cell deficient individuals. Vaccine 30:2768-77
Shi, Xin; Zhang, Ping; Sempowski, Gregory D et al. (2011) Thymopoietic and bone marrow response to murine Pneumocystis pneumonia. Infect Immun 79:2031-42
Siggins, Robert W; Melvan, John N; Welsh, David A et al. (2011) Alcohol suppresses the granulopoietic response to pulmonary Streptococcus pneumoniae infection with enhancement of STAT3 signaling. J Immunol 186:4306-13
Shi, Xin; LeCapitaine, Nicole J; Rudner, Xiaowen L et al. (2009) Lymphocyte apoptosis in murine Pneumocystis pneumonia. Respir Res 10:57

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