The Imaging Core (Core C) will be crucial for the quality and productivity of all participating projects. The Imaging Core centralizes the equipment, purchasing of supplies, training and scheduling required to complete the imaging experiments proposed in the four projects comprising this Program Project Grant. The microscopy imaging experiments that will be supported by this core include: light, fluorescence, laser scanning confocal and time-lapse confocal microscopy. Core C will provide investigators with the necessary microscopy equipment, which is already in place. In addition, Core C staff will provide the professional training required for specific imaging applications, including the use of hardware, specific imaging software, data acquisition and analysis necessary to carry out the proposed experiments accurately and in a timely manner. This will increase the efficiency and productivity of the Program Project Grant, because all personnel will be properly trained, and common techniques will be standardized. This will likewise reduce costs by eliminating duplication of efforts. Core C will also provide all of the reagents required for imaging, including fluorescent antibodies and dyes. This will eliminate waste and duplication of supplies and effort. Additionally, Core C will maintain all equipment through service contracts. This will ensure that all equipment is in proper working order when needed by individual projects, thereby reducing downtime and increasing efficiency.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Henry Ford Health System
United States
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Gordish, Kevin L; Beierwaltes, William H (2014) Resveratrol induces acute endothelium-dependent renal vasodilation mediated through nitric oxide and reactive oxygen species scavenging. Am J Physiol Renal Physiol 306:F542-50
Ortiz-Capisano, M Cecilia; Reddy, Mahendranath; Mendez, Mariela et al. (2013) Juxtaglomerular cell CaSR stimulation decreases renin release via activation of the PLC/IP(3) pathway and the ryanodine receptor. Am J Physiol Renal Physiol 304:F248-56
Atchison, Douglas K; Beierwaltes, William H (2013) The influence of extracellular and intracellular calcium on the secretion of renin. Pflugers Arch 465:59-69
Ortiz-Capisano, M Cecilia; Atchison, Douglas K; Harding, Pamela et al. (2013) Adenosine inhibits renin release from juxtaglomerular cells via an A1 receptor-TRPC-mediated pathway. Am J Physiol Renal Physiol 305:F1209-19
Ramseyer, Vanesa D; Garvin, Jeffrey L (2013) Tumor necrosis factor-ýý: regulation of renal function and blood pressure. Am J Physiol Renal Physiol 304:F1231-42
Beierwaltes, William H (2013) Endothelial dysfunction in the outer medullary vasa recta as a key to contrast media-induced nephropathy. Am J Physiol Renal Physiol 304:F31-2
Atchison, Douglas K; Harding, Pamela; Beierwaltes, William H (2013) Vitamin D increases plasma renin activity independently of plasma Ca2+ via hypovolemia and *-adrenergic activity. Am J Physiol Renal Physiol 305:F1109-17
Cabral, Pablo D; Garvin, Jeffrey L (2013) Less potassium coming out, less sodium going in: phenotyping ROMK knockout rats. Hypertension 62:240-1
Ren, Yilin; D'Ambrosio, Martin A; Wang, Hong et al. (2012) Mechanisms of carbon monoxide attenuation of tubuloglomerular feedback. Hypertension 59:1139-44
Beierwaltes, William H (2012) Are microRNAs the key to transforming renin progenitor cells in the afferent renal circulation? Am J Physiol Renal Physiol 302:F27-8

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