The Protein Biochemistry Core will provide support and expertise in protein isolation and analysis for the COBRE research project investigators, as well as other faculty at Sanford Research/USD. The core will provide support and consultation for gel-based proteomic separations, robotic spot excision from polyacrylamide gels, multiplex protein analysis, protein fractionation, semi-preparative and analytical high performance liquid chromatography (HPLC), and screening for protein-protein interactions with the novel BiolD approach. In addition, the core will provide training and maintenance for its shared equipment. The core will also provide assistance in preparation of manuscripts and funding applications as they relate to the services provided. By providing these services in-house, the core will directly interface with the investigators during project design, protocol establishment, troubleshooting and execution as well as organize any further analysis outside of the core. The purpose of this core is to support the goals of its users, namely independent scientists seeking to uncover the mechanisms of human health and disease.

Public Health Relevance

The Protein Biochemistry Core will facilitate the isolation, identification and characterization of proteins for its users. This translates into a more complete understanding of how those proteins function in both health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM103620-01A1
Application #
8465602
Study Section
Special Emphasis Panel (ZGM1-TWD-B (CB))
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
1
Fiscal Year
2013
Total Cost
$151,067
Indirect Cost
$53,275
Name
Sanford Research/Usd
Department
Type
DUNS #
050113252
City
Sioux Falls
State
SD
Country
United States
Zip Code
57104
Forred, Benjamin J; Neuharth, Skyla; Kim, Dae In et al. (2016) Identification of Redox and Glucose-Dependent Txnip Protein Interactions. Oxid Med Cell Longev 2016:5829063
Rickel, Kirby; Fang, Fang; Tao, Jianning (2016) Molecular genetics of osteosarcoma. Bone :
Kim, Dae In; Jensen, Samuel C; Noble, Kyle A et al. (2016) An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell 27:1188-96
White, Katherine A; Hutton, Scott R; Weimer, Jill M et al. (2016) Diet-induced obesity prolongs neuroinflammation and recruits CCR2(+) monocytes to the brain following herpes simplex virus (HSV)-1 latency in mice. Brain Behav Immun 57:68-78
Simpkins, Jessica A; Rickel, Kirby E; Madeo, Marianna et al. (2016) Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97
Booze, Michelle L; Hansen, Jason M; Vitiello, Peter F (2016) A novel mouse model for the identification of thioredoxin-1 protein interactions. Free Radic Biol Med 99:533-543
Yao, Qingqing; Liu, Yangxi; Tao, Jianning et al. (2016) Hypoxia-mimicking nanofibrous scaffolds promote endogenous bone regeneration. ACS Appl Mater Interfaces :
Alam, Samer G; Zhang, Qiao; Prasad, Nripesh et al. (2016) The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity. Sci Rep 6:38063
Mdaki, Kennedy S; Larsen, Tricia D; Wachal, Angela L et al. (2016) Maternal high-fat diet impairs cardiac function in offspring of diabetic pregnancy through metabolic stress and mitochondrial dysfunction. Am J Physiol Heart Circ Physiol 310:H681-92
Baack, Michelle L; Puumala, Susan E; Messier, Stephen E et al. (2016) Daily Enteral DHA Supplementation Alleviates Deficiency in Premature Infants. Lipids 51:423-33

Showing the most recent 10 out of 30 publications