Diabetic animal models are essential for studies in all of the COBRE projects and are also often required by other diabetes researchers. However, induction and monitoring of diabetes and maintenance of diabetic animals for long durations are associated with a tremendous amount of routine work. The goal of this Core is to provide a centralized service for induction of diabetes, maintenance and use of diabetic animals and to provide support to the diabetes research. In the first phase of COBRE, we have establistied the diabetic animal core and diabetic animal tissue bank. The Core has provided diabetic animals, tissues, data and technical assistance to multiple Pis. The Core has greatly increased the efficiency of diabetic animal research and has reduced the costs to PJIs and other COBRE members for diabetic animal models. As diabetes research expands in Oklahoma, there is a demand to expand this Core service to serve more diabetes researchers in Oklahoma. In the second phase of the COBRE, we will continue and augment these Core services. 1). To induce diabetes by streptozotocin injection in rats or mice or in transgenic or gene knockout mice as required by investigators. 2). To breed and genotype genetic diabetic mice and rats. 3). To monitor diabetes by measuring hyperglycemia and inject insulin when necessary. 4). To collect and record clinical data from diabetic animals, such as body weight and urine volume. 5). To monitor renal function of diabetic animals by measuring albumin and creatinine concentrations in the urine. 6). To provide special diets for diabetic animals upon request by users. 7). To induce retinal neovascularization in the oxygen-induced retinopathy (OIR) model, a commonly used model for proliferative diabetic retinopathy. 8). To perform specialized assays to evaluate diabetic complications. 9). To dissect tissues and coordinate sharing of animal tissues. This Diabetic Animal Core will assist the PJIs in their projects and reduce their routine work in the induction and maintenance of diabetic animals. The coordinated sharing of diabetic animal tissues will also substantially reduce the budget for animal models.

Public Health Relevance

Diabetes represents a major threat to the health of working age and older populations. More diabetes research is required to understand the pathogenesis of diabetes and its complications and develop new treatments. Most diabetes studies require using diabetic animal models. This core will provide centralized support to researchers using diabetic animal models and thus, will promote diabetes research.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Exploratory Grants (P20)
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Special Emphasis Panel (ZRR1)
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Oklahoma City
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Orock, Albert; Logan, Sreemathi; Deak, Ferenc (2018) Munc18-1 haploinsufficiency impairs learning and memory by reduced synaptic vesicular release in a model of Ohtahara syndrome. Mol Cell Neurosci 88:33-42
Wang, Bing; Li, Pui-Kai; Ma, Jian-Xing et al. (2018) Therapeutic Effects of a Novel Phenylphthalimide Analog for Corneal Neovascularization and Retinal Vascular Leakage. Invest Ophthalmol Vis Sci 59:3630-3642
Shin, Younghwa; Moiseyev, Gennadiy; Petrukhin, Konstantin et al. (2018) A novel RPE65 inhibitor CU239 suppresses visual cycle and prevents retinal degeneration. Biochim Biophys Acta Mol Basis Dis 1864:2420-2429
Fu, Shuhua; Dong, Shuqian; Zhu, Meili et al. (2018) VEGF as a Trophic Factor for Müller Glia in Hypoxic Retinal Diseases. Adv Exp Med Biol 1074:473-478
Chen, Qian; Qiu, Fangfang; Zhou, Kelu et al. (2017) Pathogenic Role of microRNA-21 in Diabetic Retinopathy Through Downregulation of PPAR?. Diabetes 66:1671-1682
Du, Mei; Martin, Ashley; Hays, Franklin et al. (2017) Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4. Mol Vis 23:185-197
Malechka, Volha V; Moiseyev, Gennadiy; Takahashi, Yusuke et al. (2017) Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy. Am J Pathol 187:2222-2231
Qiu, Fangfang; Liu, Zhen; Zhou, Yueping et al. (2017) Decreased Circulating Levels of Dickkopf-1 in Patients with Exudative Age-related Macular Degeneration. Sci Rep 7:1263
Pearsall, Elizabeth A; Cheng, Rui; Zhou, Kelu et al. (2017) PPAR? is essential for retinal lipid metabolism and neuronal survival. BMC Biol 15:113
Le, Yun-Zheng (2017) VEGF production and signaling in Müller glia are critical to modulating vascular function and neuronal integrity in diabetic retinopathy and hypoxic retinal vascular diseases. Vision Res 139:108-114

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