The UCLA Cytometry Core Facility (CCF), a campus-wide resource, provides state-of-the-art instrumentation and expertise at an affordable cost to UCLA researchers needing flow and ELISpot image cytometry. In 1988 the Jonsson Comprehensive Cancer Center established the resource, and since 1991 the Center For AIDS Research (CFAR) has provided additional support. Since 2000 Dr. Jamieson, an accomplished cellular immunologist, directs the CCF and is available to users for consultation. Ms. Schmid, the manager of the facility since 1988, is responsible for the CCF's daily operation and assists Dr. Jamieson with long-term planning. Ms. Schmid is an expert flow cytometrist, well known for her development of flow cytometry methods and her pivotal role in establishing biosafety guidelines for cell sorting. Since 2001 the CCF provides ELISpot assay services under the guidance of Dr. Uittenbogaart, a noted cellular immunologist with extensive expertise in cytokine measurements. The CCF has a single-laser FACScan, a dual-laser FACSCalibur, a three-laser BD LSR I, a modified, dual-laser, five-color FACScan, three, three-laser sorters, an autoMACS magnetic separator, and an off-line data analysis computer. An ImmunoSpot Analyzer, three, free-standing analyzers, and five biological safety cabinets are also available. Cytometric instrumentation is expensive and beyond the budget of most individual researchers. The CCF is easily accessible and is responsible for procurement, daily calibration, maintenance and repair of all instrumentation. CCF personnel have a combined 82 years of flow cytometry and 46 years of cytokine measurement experience. Expertise is available to investigators through one-on-one consultation, classes, a website, poster presentations, and open houses. Ms. Schmid and Dr. Uittenbogaart also work with researchers to adapt or develop assays to enable investigators to pursue research questions in novel ways. CCF services help researchers with varying amounts of experience in cytometry to perform high quality assays with minimal time expenditure. Workshops, seminars, and utilization of new assays ensure that researchers remain on the cutting edge of cytometry without investing their own funds or staff time on keeping current in the latest advances. During the last funding period a total of 250 investigators used the CCF;40 of those were CFAR/AIDS Institute members representing 38% of use. This documents the role of the CCF in enhancing HIV research at UCLA with its potential contribution to improving the health of HIV-infected patients.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Center Core Grants (P30)
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Special Emphasis Panel (ZAI1-EC-A)
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University of California Los Angeles
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Qi, Hangfei; Chu, Virginia; Wu, Nicholas C et al. (2017) Systematic identification of anti-interferon function on hepatitis C virus genome reveals p7 as an immune evasion protein. Proc Natl Acad Sci U S A 114:2018-2023
Graham, Nicholas A; Minasyan, Aspram; Lomova, Anastasia et al. (2017) Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. Mol Syst Biol 13:914
Castaneda, Julie T; Harui, Airi; Roth, Michael D (2017) Regulation of Cell Surface CB2 Receptor during Human B Cell Activation and Differentiation. J Neuroimmune Pharmacol 12:544-554
Harawa, Nina T; Holloway, Ian W; Leibowitz, Arleen et al. (2017) Serious concerns regarding a meta-analysis of preexposure prophylaxis use and STI acquisition. AIDS 31:739-740
Richardson-Harman, Nicola; Parody, Robert; Anton, Peter et al. (2017) Analytical Advances in the Ex Vivo Challenge Efficacy Assay. AIDS Res Hum Retroviruses 33:395-403
Foo, Suan-Sin; Chen, Weiqiang; Chan, Yen et al. (2017) Asian Zika virus strains target CD14+ blood monocytes and induce M2-skewed immunosuppression during pregnancy. Nat Microbiol 2:1558-1570
Bergstrom, K; Fu, J; Johansson, M E V et al. (2017) Core 1- and 3-derived O-glycans collectively maintain the colonic mucus barrier and protect against spontaneous colitis in mice. Mucosal Immunol 10:91-103
Farmer, Shu; Mindry, Deborah; Comulada, W Scott et al. (2017) Mobile Phone Ecological Momentary Assessment of Daily Stressors Among People Living With HIV: Elucidating Factors Underlying Health-Related Challenges in Daily Routines. J Assoc Nurses AIDS Care 28:737-751
Tan, Diane; Holloway, Ian W; Gildner, Jennifer et al. (2017) Alcohol Use and HIV Risk Within Social Networks of MSM Sex Workers in the Dominican Republic. AIDS Behav 21:216-227
Bristow, Claire C; Lee, Sung-Jae; Severe, Linda et al. (2017) Attributes of diagnostic tests to increase uptake of dual testing for syphilis and HIV in Port-au-Prince, Haiti. Int J STD AIDS 28:259-264

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