Due to outstanding progress and important developments in the field of chimeric mouse models reconstituted with human immune systems and cells, Core H, the Small Animal Biocontainment (ABC) Core expanded its scope and now includes two sites. Site A is located at the Massachusetts General Hospital (MGH), and Site B is the existing BL-3 ABC Facility at the Dana-Farber Cancer Institute (DFCI). Site A will provide the following services: 1. Generation of humanized BLT mice (NOD-SCID-Hu mice transplanted wit human fetal thymus, liver and hematopoietic stems cells (HSC)) for CFAR investigators to use for experiments, to be performed at the MGH or DFCI Sites of this core, or at the site of the collaborating investigator, following transfer of the BLT mice to his/her animal facility. 2. Bleeding and flow cytometry of peripheral blood leukocytes of the BLT mice to characterize their human immune reconstitution prior to use by collaborating investigators. 3. For collaborating investigators choosing to perform their experiments at the MGH site, support in the handling and analysis of HIV-infected mice. 4. For collaborating investigators choosing to perform experiments in their own animal facilities, training in the safe handling of HIV-infected mice. 5. Further characterization of the human virus-specific cellular and humoral immune responses generated in HIV-infected BLT mice, to expand the areas of investigation this model can support. Site B will provide the following services: 1. The use of an animal facility in which the spread of contagious agents is prevented between animals. 2. Technical support in handling animals potentially infectious to humans or animals. 3. Access to technical support for performing HIV-1 inoculations into chimeric BLT or NOG (i.e., NOD/SCID.IL-2RYNull) mice reconstituted with CD34+ human HSC, which will be housed to evaluate candidate antiviral agents for efficacy and toxicity in these novel small animal models. A special plus of Site B is the ability to permit work with Mycobacterium tuberculosis (MTb) or HIV-1/MTb co-infections. 4. The use of safe working conditions. 5. Training for safe handling of potential human pathogens in small animal models.

Public Health Relevance

Core H will allow CFAR investigators to study HIV immunopathogenesis and host-virus dynamics in small animal models, which can also support coinfection with HIV-1 and Mycobacterium tuberculosis, two pathogens that threaten public health worldwide.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI060354-09
Application #
8380987
Study Section
Special Emphasis Panel (ZAI1-SV-A)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
9
Fiscal Year
2012
Total Cost
$324,721
Indirect Cost
$59,261
Name
Harvard University
Department
Type
DUNS #
082359691
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Hyle, Emily P; Mayosi, Bongani M; Middelkoop, Keren et al. (2017) The association between HIV and atherosclerotic cardiovascular disease in sub-Saharan Africa: a systematic review. BMC Public Health 17:954
Luckow, Peter W; Kenny, Avi; White, Emily et al. (2017) Implementation research on community health workers' provision of maternal and child health services in rural Liberia. Bull World Health Organ 95:113-120
McCluskey, Suzanne M; Boum 2nd, Yap; Musinguzi, Nicholas et al. (2017) Brief Report: Appraising Viral Load Thresholds and Adherence Support Recommendations in the World Health Organization Guidelines for Detection and Management of Virologic Failure. J Acquir Immune Defic Syndr 76:183-187
Viganò, Selena; Negrón, Jordi J; Tse, Samantha et al. (2017) HLA-G+ HIV-1-specific CD8+ T cells are associated with HIV-1 immune control. AIDS 31:207-212
Brown, Danielle N; Langan, Sinéad M; Freeman, Esther E (2017) Task Shifting in Dermatology: A Call to Action. JAMA Dermatol 153:1179-1180
Calabrese, Sarah K; Underhill, Kristen; Mayer, Kenneth H (2017) HIV Preexposure Prophylaxis and Condomless Sex: Disentangling Personal Values From Public Health Priorities. Am J Public Health 107:1572-1576
Janjua, Sumbal A; Staziaki, Pedro V; Szilveszter, Balint et al. (2017) Presence, Characteristics, and Prognostic Associations of Carotid Plaque Among People Living With HIV. Circ Cardiovasc Imaging 10:
Manickam, Cordelia; Martinot, Amanda J; Jones, Rhianna A et al. (2017) Hepatic immunopathology during occult hepacivirus re-infection. Virology 512:48-55
Levison, Julie H; Bogart, Laura M; Khan, Iman F et al. (2017) ""Where It Falls Apart"": Barriers to Retention in HIV Care in Latino Immigrants and Migrants. AIDS Patient Care STDS 31:394-405
Mayer, Kenneth H; Jones, Daniel; Oldenburg, Catherine et al. (2017) Optimal HIV Postexposure Prophylaxis Regimen Completion With Single Tablet Daily Elvitegravir/Cobicistat/Tenofovir Disoproxil Fumarate/Emtricitabine Compared With More Frequent Dosing Regimens. J Acquir Immune Defic Syndr 75:535-539

Showing the most recent 10 out of 944 publications