Until recently, we supported three separate shared services that provided various methods for the qualitative or quantitative analysis of macromolecules, including Darkroom, Radioisotope Counting, and Flow Cytometry Facilities. In the last five years, we have taken advantage of new technologies in this area through the purchase of phosphorimagers and automated DNA sequencers. We have combined all of these resources into the Macromolecular Analysis Facility in this application because of their common objectives and to increase the efficiency of their operation. The goal of this shared service is to provide researchers in the Center with access to essential, well-maintained analytical instruments and training in their use. Individual investigators are responsible for sample preparation and, with the exception of DNA sequencing, the operation of the instrument.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA007175-34
Application #
6268574
Study Section
Project Start
1998-05-22
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
34
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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Liu, Ning; Tian, Ru; Loeb, Daniel D (2003) Base pairing among three cis-acting sequences contributes to template switching during hepadnavirus reverse transcription. Proc Natl Acad Sci U S A 100:1984-9
Mueller-Hill, Karlyn; Loeb, Daniel D (2002) cis-Acting sequences 5E, M, and 3E interact to contribute to primer translocation and circularization during reverse transcription of avian hepadnavirus DNA. J Virol 76:4260-6

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