PROJECT SUIVIMARY (See instructions): This Case CCC Cytometry &Imaging Microscopy Core provides access to instrumentation, instrumentation specific training, experimental consultation, data analysis, and data analysis training. By special request, the Core provides technical services preliminary to measurement such as cell culture, fixation, staining, etc. The Core covers flow cytometry, laser scanning cytometry, imaging flow cytometry, fluorescence-activated cell sorting (FACS), automated live-dead cell counting, confocal laser scanning microscopy, fluorescence video microscopy, and bright field automated whole slide imaging and has up-to-date analytical software for each activity. These services are broad-based, providing fundamental tools for basic sciences. The Core has two sites on the CWRU campus and another site through an alliance with the Case Center for AIDS Research (CFAR). The services are: 1) user access to instruments, 2) staff assisted use of instruments, and 3) performed by staff. The user base consists largely of Case CCC members from all the Research Programs with members from the Hematopoietic Disorders, Developmental Therapeutics, and Cancer Imaging Programs being the heaviest users. Overall, this Core provides fundamental services that may or may not be a large part of any one particular paper or grant but support the research efforts of a well-funded and highly published group of investigators. Services such as cell counting, cell sorting, or simple immunofluorescence analysis may or may not make it into a publication even though they are used on a daily basis to maintain cell lines, quality control cells, or to provide analysis that impacts the direction of research represented by publications and grants. The Core has been critical in studies that: provided a mechanism-based rationale for the development of HDAC and HAUSP inhibitors for combined use in cancer therapy;elucidated the mechanisms that initiate and maintain OIS in epithelial cells;and determining the inhibitory role for KLF4 during breast cancer metastasis.

Public Health Relevance

The Case Comprehensive Cancer Center is Northeast Ohio's only NCI designated comprehensive cancer center providing bench-to-bedside medical research involving partnerships between basic, clinical and population scientists to speed translation of laboratory discoveries into new prevention/intervention and cancer treatments.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA043703-23
Application #
8484954
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-08-01
Project End
2018-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
23
Fiscal Year
2013
Total Cost
$200,587
Indirect Cost
$73,428
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Zhao, S; Sedwick, D; Wang, Z (2015) Genetic alterations of protein tyrosine phosphatases in human cancers. Oncogene 34:3885-94
Dermawan, Josephine Kam Tai; Gurova, Katerina; Pink, John et al. (2014) Quinacrine overcomes resistance to erlotinib by inhibiting FACT, NF-?B, and cell-cycle progression in non-small cell lung cancer. Mol Cancer Ther 13:2203-14
Brubaker, Douglas; Difeo, Analisa; Chen, Yanwen et al. (2014) Drug Intervention Response Predictions with PARADIGM (DIRPP) identifies drug resistant cancer cell lines and pathway mechanisms of resistance. Pac Symp Biocomput :125-35
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Zapanta Rinonos, Serendipity; Rai, Urvashi; Vereb, Sydney et al. (2014) Sequential logic of polarity determination during the haploid-to-diploid transition in Saccharomyces cerevisiae. Eukaryot Cell 13:1393-402
Sizemore, Gina M; Sizemore, Steven T; Seachrist, Darcie D et al. (2014) GABA(A) receptor pi (GABRP) stimulates basal-like breast cancer cell migration through activation of extracellular-regulated kinase 1/2 (ERK1/2). J Biol Chem 289:24102-13
Sossey-Alaoui, Khalid; Pluskota, Elzbieta; Davuluri, Gangarao et al. (2014) Kindlin-3 enhances breast cancer progression and metastasis by activating Twist-mediated angiogenesis. FASEB J 28:2260-71
Dotan, Efrat; Devarajan, Karthik; D'Silva, A James et al. (2014) Patterns of use and tolerance of anti-epidermal growth factor receptor antibodies in older adults with metastatic colorectal cancer. Clin Colorectal Cancer 13:192-8
Arachiche, Amal; de la Fuente, MarĂ­a; Nieman, Marvin T (2014) Platelet specific promoters are insufficient to express protease activated receptor 1 (PAR1) transgene in mouse platelets. PLoS One 9:e97724

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